Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
(→Pre-existing parts we improved) |
(→Pre-existing parts we improved) |
||
Line 78: | Line 78: | ||
== Pre-existing parts we improved == | == Pre-existing parts we improved == | ||
- | - [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - | + | - [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - '''The Berkeley Lysis Device''' BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments. |
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Revision as of 01:28, 22 September 2011
Data
team, please check this page: [1]
Contents |
New parts
we must have 3 favourite, then the others come in a "other biobrick" section
Variants without additional lac operator (constitutive):
[http://partsregistry.org/Part:BBa_K613001 K613001]
[http://partsregistry.org/Part:BBa_K613002 K613002]
[http://partsregistry.org/Part:BBa_K613003 K613003]
[http://partsregistry.org/Part:BBa_K613004 K613004]
[http://partsregistry.org/Part:BBa_K613005 K613005]
[http://partsregistry.org/Part:BBa_K613006 K613006]
Variants with additional lac operator (LacI repressed):
[http://partsregistry.org/Part:BBa_K613007 K613007]
[http://partsregistry.org/Part:BBa_K613008 K613008]
[http://partsregistry.org/Part:BBa_K613009 K613009]
[http://partsregistry.org/Part:BBa_K613010 K613010]
[http://partsregistry.org/Part:BBa_K613011 K613011]
[http://partsregistry.org/Part:BBa_K613012 K613012]
Medium-strength Plac
[http://partsregistry.org/Part:BBa_K613000 K613000]
TetR Mutants
TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
---|---|---|---|---|---|---|---|---|---|
E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | v | BBa_K613016 | v | v |
Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v |
T7 promoter variants
Pre-existing parts we improved
- [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - The Berkeley Lysis Device BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.