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Team:Freiburg/Notebook/20 June
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/19_June">Previous entry</a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 20 June </a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/21_June">Next entry</a> | ||
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
===Lysis cassette test=== | ===Lysis cassette test=== | ||
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5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed. | 5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed. | ||
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==Digestion of PR1-PR9== | ==Digestion of PR1-PR9== | ||
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Enzyme 2: PstI<br/> | Enzyme 2: PstI<br/> | ||
Samples were then loaded onto a gel. | Samples were then loaded onto a gel. | ||
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Latest revision as of 01:21, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
