Team:Freiburg/Notebook/28 August

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/27_August">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 28 August </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/29_August">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===M48+GFP===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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We started with a 3A assembly for the correct temperature sensitive promoter with GFP in order to test it.
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<br>
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Everything was done according to SOPs...
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<br>
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After 2 days there were no colonies...
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<br>
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Sophie decided to try it herself, since depression would start to grow big in me...
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Latest revision as of 01:12, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Testdigest

Investigators: Sophie, Sandra

Repeat of the testdigest of the ligated parts:

  • Lovtap
  • Not-Gate
  • pSB1A3

The expected size of the bands should be 2400bp and about 2000bp.

Miniprep

Investigators: Sandra

Miniprep of new 3A-assembly.
Samples were loaded onto a gel to verify the size of the samples.
The expected size: 4000bp.

The bands showed only a size of 1500bp.

The 3A-assembly did not work. Probably because of the Lovtap, since the sequencing of the Not-Gate was positive.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

M48+GFP

Investigators:Theo

We started with a 3A assembly for the correct temperature sensitive promoter with GFP in order to test it.


Everything was done according to SOPs...


After 2 days there were no colonies...


Sophie decided to try it herself, since depression would start to grow big in me...

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/28_August"