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Team:Freiburg/Notebook/28 August
From 2011.igem.org
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{{:Team:Freiburg/Templates/header}} | {{:Team:Freiburg/Templates/header}} | ||
| + | <html> | ||
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/27_August">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 28 August </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/29_August">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
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==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
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*Not-Gate | *Not-Gate | ||
*pSB1A3 | *pSB1A3 | ||
| + | |||
| + | The expected size of the bands should be 2400bp and about 2000bp. | ||
===Miniprep=== | ===Miniprep=== | ||
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Samples were loaded onto a gel to verify the size of the samples.<br/> | Samples were loaded onto a gel to verify the size of the samples.<br/> | ||
The expected size: 4000bp. | The expected size: 4000bp. | ||
| + | |||
| + | The bands showed only a size of 1500bp. | ||
| + | |||
| + | The 3A-assembly did not work. | ||
| + | Probably because of the Lovtap, since the sequencing of the Not-Gate was positive. | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
| - | === | + | ===M48+GFP=== |
| - | '''Investigators: | + | '''Investigators:Theo''' |
| + | |||
| + | We started with a 3A assembly for the correct temperature sensitive promoter with GFP in order to test it. | ||
| + | |||
| + | <br> | ||
| + | |||
| + | Everything was done according to SOPs... | ||
| + | |||
| + | <br> | ||
| + | |||
| + | After 2 days there were no colonies... | ||
| + | <br> | ||
| + | Sophie decided to try it herself, since depression would start to grow big in me... | ||
==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== | ||
Latest revision as of 01:12, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
