Team:Freiburg/Notebook/26 August

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/25_August">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 26 August </a>
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==Meeting==
==Meeting==
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===repeating quickchange PCR===
-
'''Investigators:NAME'''
+
'''Investigators:Julia'''
 +
<br/>
 +
repeating PCR with fresh DNA template.
 +
<br/>
 +
digest with DpnI,added directly to the PCR tube.<br/> After NEB the DPNI has also a good activity in Phusion HF buffer.<br/>
 +
==<span style="color:blue;">blue light receptor</span>==
 +
===Ligation===
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'''Investigators: Sophie'''<br/>
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Date: 25.08.11<br/>
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continue from experiment: Digestion; 
 +
Investigator: Sandra<br/> 
 +
Project Name: Blue light receptor<br/>
 +
all samples stored in Blue light box
-
==<span style="color:blue;">blue light receptor</span>==
 
-
===NAME OF YOUR EXPERIMENT===
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===Transformation===
-
Transformation
+
-
Name: Sophie
+
-
Date: 26.08.11
+
-
Continue from                                    Date: 26.08.11        Name: Sophie               
+
-
Experiment Ligation   
+
-
Project Name: Blue light receptor
+
-
Procedure
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'''Investigators: Sophie'''<br/>
 +
Date: 26.08.11<br/>
 +
Continue from                                  Date: 26.08.11        Name: Sophie               
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Experiment Ligation <br/> 
 +
Project Name: Blue light receptor<br/>
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Procedure
 +
<br/>
1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
2. thaw cells on ice 20 minutes
2. thaw cells on ice 20 minutes
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9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
-
 
+
<br/>
Documentation:
Documentation:
Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
-
Why? The last cloning was done with a NOT-PCR-Product which might make problems because it does not have enough bases after the restriciton sites.
+
Why? The last cloning was done with a NOT-PCR-Product which might make problems because it does not have enough bases after the restriciton sites.<br/>
-
Name of the samples: Not 1:1, Not 1:3, nOt 1:1 nOt 1:3, noT 1:1, noT 1: 3
+
Name of the samples: Not 1:1, Not 1:3, nOt 1:1 nOt 1:3, noT 1:1, noT 1: 3<br/>
-
stored in incubator on Amp plates
+
stored in incubator on Amp plates<br/>
-
vector: psb1A3
+
vector: psb1A3<br/>
-
inserts: Not/ nOt/ noT + LOV-3A-PCR
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inserts: Not/ nOt/ noT + LOV-3A-PCR<br/>
-
 
+
-
 
+
-
 
+
-
'''Investigators:NAME'''
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==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
-
===NAME OF YOUR EXPERIMENT===
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===New 2A assembly sequencing results===
-
'''Investigators:NAME'''
+
'''Investigators:Theo'''
 +
Results came in: [[File:S4+S15 no phosphatase.gb]]... The Part is finally there.. Stock was made
 +
As I found out during the next days the stock was not correct.. so the elusive part was again lost..
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Latest revision as of 01:12, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

Meeting

attendants:
Time:

green light receptor

already done:


To-do:

blue light receptor

already done:


To-do:

red light receptor

already done:


To-do:

Lysis cassette

already done:


To-do:


Precipitator

already done:


To-do:

green light receptor

repeating quickchange PCR

Investigators:Julia
repeating PCR with fresh DNA template.
digest with DpnI,added directly to the PCR tube.
After NEB the DPNI has also a good activity in Phusion HF buffer.

blue light receptor

Ligation

Investigators: Sophie
Date: 25.08.11
continue from experiment: Digestion; Investigator: Sandra
Project Name: Blue light receptor
all samples stored in Blue light box


Transformation

Investigators: Sophie
Date: 26.08.11
Continue from Date: 26.08.11 Name: Sophie Experiment Ligation
Project Name: Blue light receptor

Procedure
1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells) 2. thaw cells on ice 20 minutes 3. pipette 50 μl cells and 2 μl DNA into eppi still on ice! 4. Incubate for 30 minutes on ice 5. Heat at 42°C for 60 sec 6. Incubate on ice for 5 minutes 7. Add 200 μl LB Broth 8. Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!) 9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance


Documentation: Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc. Why? The last cloning was done with a NOT-PCR-Product which might make problems because it does not have enough bases after the restriciton sites.

Name of the samples: Not 1:1, Not 1:3, nOt 1:1 nOt 1:3, noT 1:1, noT 1: 3

stored in incubator on Amp plates

vector: psb1A3
inserts: Not/ nOt/ noT + LOV-3A-PCR

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

New 2A assembly sequencing results

Investigators:Theo


Results came in: File:S4+S15 no phosphatase.gb... The Part is finally there.. Stock was made As I found out during the next days the stock was not correct.. so the elusive part was again lost..

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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