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Team:Freiburg/Notebook/25 August
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==Commons== | ==Commons== | ||
Latest revision as of 01:12, 22 September 2011
Contact 
Contents |
Commons
PCR of pSB1A3 and pSB1C3
Investigators: Sandra
Primer:
- P35
- P36
Testdigest:
Expected bands:
pSB1A3: 2155 bp
pSB1C3: 2070 bp
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Miniprep
Investigators: Sandra
Miniprep of LovTAP-NotGate-pSB1A3.
After Miniprep the samples were digested with:
- EcoRI
- PstI
The testdigest showed no bands!
Trafo
Investigators: Sandra
Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick. Incubation over night.
Clones need to be picked tomorrow.
3A-assembly:Digestion
Investigators: Sandra
Digestion of:
- Not
- nOt
- noT
- Lovtap 3A
- pSB1A3
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Insertion of the precipitator into a iGEM vector
Investigators: Julia
The precipitator was cloned into pSB1K3 and pSB1T3.
Miniprep
Investigators: Sandra
continue from Transformation
22.08.11
Investigator: Sophie
Project name: inducible promoter for pbd
Testdigest
Investigators: Sophie
continue from Miniprep
Investigatora: Sandra
project name: inducible promoter for pbd
