Team:Freiburg/Notebook/25 August

From 2011.igem.org

(Difference between revisions)
(→Trafo)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Freiburg/Templates/header}}
{{:Team:Freiburg/Templates/header}}
 +
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/24_August">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 25 August </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/26_August">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
 +
 +
 +
==Commons==
 +
 +
===PCR of pSB1A3 and pSB1C3===
 +
 +
'''Investigators: Sandra'''
 +
 +
Primer:
 +
*P35
 +
*P36
 +
 +
Testdigest:
 +
 +
Expected bands:<br/>
 +
pSB1A3: 2155 bp
 +
 +
pSB1C3: 2070 bp
==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
Line 6: Line 37:
'''Investigators:NAME'''
'''Investigators:NAME'''
-
 
-
 
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
Line 23: Line 52:
*PstI
*PstI
 +
The testdigest showed no bands!
===Trafo===
===Trafo===
Line 29: Line 59:
Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick.
Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick.
 +
Incubation over night.
 +
Clones need to be picked tomorrow.
===3A-assembly:Digestion===
===3A-assembly:Digestion===
Line 63: Line 95:
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===Insertion of the precipitator into a iGEM vector===
-
'''Investigators: NAME'''
+
'''Investigators: Julia'''
 +
<br/>
 +
The precipitator was cloned into pSB1K3 and pSB1T3.
 +
 
 +
===Miniprep===
 +
'''Investigators: Sandra'''
 +
<br/>
 +
continue from Transformation
 +
<br/>
 +
22.08.11
 +
<br/>
 +
Investigator: Sophie
 +
<br/>
 +
Project name: inducible promoter for pbd
 +
 
 +
===Testdigest===
 +
'''Investigators: Sophie
 +
<br/>
 +
continue from Miniprep
 +
<br/>
 +
Investigatora: Sandra
 +
<br/>
 +
project name: inducible promoter for pbd
 +
[[File:Freiburg25.8.11.jpg]]
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:12, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

Commons

PCR of pSB1A3 and pSB1C3

Investigators: Sandra

Primer:

  • P35
  • P36

Testdigest:

Expected bands:
pSB1A3: 2155 bp

pSB1C3: 2070 bp

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep

Investigators: Sandra


Miniprep of LovTAP-NotGate-pSB1A3.

After Miniprep the samples were digested with:

  • EcoRI
  • PstI

The testdigest showed no bands!

Trafo

Investigators: Sandra

Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick. Incubation over night.

Clones need to be picked tomorrow.

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

  • Not
  • nOt
  • noT
  • Lovtap 3A
  • pSB1A3


red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Insertion of the precipitator into a iGEM vector

Investigators: Julia
The precipitator was cloned into pSB1K3 and pSB1T3.

Miniprep

Investigators: Sandra
continue from Transformation
22.08.11
Investigator: Sophie
Project name: inducible promoter for pbd

Testdigest

Investigators: Sophie
continue from Miniprep
Investigatora: Sandra
project name: inducible promoter for pbd Freiburg25.8.11.jpg

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/25_August"