Team:Freiburg/Notebook/25 August

From 2011.igem.org

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/24_August">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 25 August </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/26_August">Next entry</a>
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==Commons==
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===PCR of pSB1A3 and pSB1C3===
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'''Investigators: Sandra'''
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Primer:
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*P35
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*P36
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Testdigest:
 +
 +
Expected bands:<br/>
 +
pSB1A3: 2155 bp
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pSB1C3: 2070 bp
==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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'''Investigators:NAME'''
'''Investigators:NAME'''
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==<span style="color:blue;">blue light receptor</span>==
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===Miniprep===
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==<span style="color:blue;">blue light receptor</span>==
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'''Investigators: Sandra'''
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===NAME OF YOUR EXPERIMENT===
 
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'''Investigators:NAME'''
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Miniprep of LovTAP-NotGate-pSB1A3.
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After Miniprep the samples were digested with:
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*EcoRI
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*PstI
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The testdigest showed no bands!
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===Trafo===
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'''Investigators: Sandra'''
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Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick.
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Incubation over night.
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Clones need to be picked tomorrow.
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===3A-assembly:Digestion===
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'''Investigators: Sandra'''
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Digestion of:
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*Not
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*nOt
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*noT
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*Lovtap 3A
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*pSB1A3
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<br/>
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==
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==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
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===NAME OF YOUR EXPERIMENT===
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===Insertion of the precipitator into a iGEM vector===
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'''Investigators: Julia'''
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<br/>
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The precipitator was cloned into pSB1K3 and pSB1T3.
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===Miniprep===
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'''Investigators: Sandra'''
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<br/>
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continue from Transformation
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<br/>
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22.08.11
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<br/>
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Investigator: Sophie
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<br/>
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Project name: inducible promoter for pbd
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'''Investigators: NAME'''
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===Testdigest===
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'''Investigators: Sophie
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<br/>
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continue from Miniprep
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<br/>
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Investigatora: Sandra
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<br/>
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project name: inducible promoter for pbd
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[[File:Freiburg25.8.11.jpg]]
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:12, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

Commons

PCR of pSB1A3 and pSB1C3

Investigators: Sandra

Primer:

  • P35
  • P36

Testdigest:

Expected bands:
pSB1A3: 2155 bp

pSB1C3: 2070 bp

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep

Investigators: Sandra


Miniprep of LovTAP-NotGate-pSB1A3.

After Miniprep the samples were digested with:

  • EcoRI
  • PstI

The testdigest showed no bands!

Trafo

Investigators: Sandra

Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick. Incubation over night.

Clones need to be picked tomorrow.

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

  • Not
  • nOt
  • noT
  • Lovtap 3A
  • pSB1A3


red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Insertion of the precipitator into a iGEM vector

Investigators: Julia
The precipitator was cloned into pSB1K3 and pSB1T3.

Miniprep

Investigators: Sandra
continue from Transformation
22.08.11
Investigator: Sophie
Project name: inducible promoter for pbd

Testdigest

Investigators: Sophie
continue from Miniprep
Investigatora: Sandra
project name: inducible promoter for pbd Freiburg25.8.11.jpg

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/25_August"