Team:Freiburg/Notebook/18 July

From 2011.igem.org

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==<span style="color:green;">green light receptor</span>==
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{{:Team:Freiburg/Templates/header}}
 +
<html>
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<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/16_July">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 18 July </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/19_July">Next entry</a>
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</div>
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</div>
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</html>
-
===NAME OF YOUR EXPERIMENT===
+
==<span style="color:grey;">Precipitator</span>==
-
'''Investigators:NAME'''
+
===PCR===
-
==<span style="color:blue;">blue light receptor</span>==
+
{| style="border-spacing:0;"
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name:
-
===NAME OF YOUR EXPERIMENT===
+
Ruediger
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date:
-
'''Investigators:NAME'''
+
18.07.2011
 +
|-
 +
| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Experiment (Date)PCR 1507
 +
(Name) Ruediger
 +
|-
 +
| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name:
-
==<span style="color:red;">red light receptor</span>==
+
GFP Pbd
-
===NAME OF YOUR EXPERIMENT===
+
|}
 +
PCR-Mixture for one Reaction:
-
'''Investigators:NAME'''
+
For a 50 µl reaction use
 +
{| style="border-spacing:0;"
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 32,5µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| H<sub>2</sub>0
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
 +
|-
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 10µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 5x Phusion Buffer
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
-
==<span style="color:orange;">Lysis cassette</span>==
+
|-
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer fw
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
-
===NAME OF YOUR EXPERIMENT===
+
|-
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer dw
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
-
'''Investigators:NAME'''
+
|-
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| dNTPs
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
 +
|-
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA-Template
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
 +
|-
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 0.5 µl
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Phusion (add in the end)
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
-
==<span style="color:grey;">Precipitator</span>==
+
|}
 +
What program do you use?
 +
 
 +
10x (95C-41C/47C/52C-72C) + 25x ((95C-60C-72C)
 +
 
 +
 
 +
How did you label the PCR-Product, where is it stored and what do you do next?
 +
 
 +
 
 +
Reactions:
 +
 
 +
P28+P18+M14.1
 +
 
 +
P28+P19+M14.1
 +
 
 +
P28+P20+M14.1
 +
 
 +
P28+P2+M14.1
 +
 
 +
P28+P3+M14.1
 +
 
 +
 
 +
P1+P18+M14.1
 +
 
 +
P1+P19+M14.1
 +
 
 +
P1+P20+M14.1
 +
 
 +
P1+P2+M14.1
 +
 
 +
P1+P3+M14.1
 +
 
 +
 
 +
 
 +
P1+P3+M14.2
 +
 
 +
 
 +
[[File:Freiburg11_Phusion_ladder.JPG]]
 +
 
 +
 
 +
[[File:Freiburg11_7_19_2011_7_31_40_PM.Jpg]]
 +
 
 +
'''Upper panel:'''All lanes at 41C
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
 +
 
 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P2+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| P1+P2+M14.1
 +
 
 +
 
 +
 
 +
 
 +
|}
 +
'''Lower Panel'''
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
 +
 
 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.1
 +
 
 +
41C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.1
 +
 
 +
47C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.2
 +
 
 +
52C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P3+M14.1
 +
 
 +
52C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P3+M14.1
 +
 
 +
41C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P18+M14.1
 +
 
 +
41C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P3+M14.1
 +
 
 +
47C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P3+M14.1
 +
 
 +
52C
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"|
 +
 
 +
|}
 +
 
 +
 
 +
[[File:Freiburg11 7 19 2011 7 31 41 PM.Jpg]]
 +
 
 +
'''Upper Panel: '''all at 47C
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
 +
 
 +
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P2+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| P1+P2+M14.1
 +
 
 +
|}
 +
'''Lower Panel:''' all at 52C
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 1'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 2'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 3'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 4'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 5'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 7'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 8'''
 +
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 9'''
 +
| style="border:0.0007in solid #000000;padding:0.0382in;"| '''Lane 10'''
-
===NAME OF YOUR EXPERIMENT===
+
|-
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Quick Load Marker
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P28+P2+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"|
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P18+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P19+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| P1+P20+M14.1
 +
| style="border-top:none;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| P1+P2+M14.1
-
'''Investigators: NAME'''
+
|}
 +
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 00:58, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Precipitator

PCR

Name:

Ruediger

Date:

18.07.2011

Continue from Experiment (Date)PCR 1507

(Name) Ruediger

Project Name:

GFP Pbd

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw
2.5µl Primer dw
1µl dNTPs
1µl DNA-Template
0.5 µl Phusion (add in the end)

What program do you use?

10x (95C-41C/47C/52C-72C) + 25x ((95C-60C-72C)


How did you label the PCR-Product, where is it stored and what do you do next?


Reactions:

P28+P18+M14.1

P28+P19+M14.1

P28+P20+M14.1

P28+P2+M14.1

P28+P3+M14.1


P1+P18+M14.1

P1+P19+M14.1

P1+P20+M14.1

P1+P2+M14.1

P1+P3+M14.1


P1+P3+M14.2


Freiburg11 Phusion ladder.JPG


Freiburg11 7 19 2011 7 31 40 PM.Jpg

Upper panel:All lanes at 41C

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P28+P18+M14.1 P28+P19+M14.1 P28+P20+M14.1 P28+P2+M14.1 P1+P19+M14.1 P1+P20+M14.1 P1+P2+M14.1



Lower Panel


Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P1+P3+M14.1

41C

P1+P3+M14.1

47C

P1+P3+M14.2

52C

P1+P3+M14.1

52C

P28+P3+M14.1

41C

P1+P18+M14.1

41C

P28+P3+M14.1

47C

P28+P3+M14.1

52C


Freiburg11 7 19 2011 7 31 41 PM.Jpg

Upper Panel: all at 47C


Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P28+P18+M14.1 P28+P19+M14.1 P28+P20+M14.1 P28+P2+M14.1 P1+P18+M14.1 P1+P19+M14.1 P1+P20+M14.1 P1+P2+M14.1

Lower Panel: all at 52C


Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10
Quick Load Marker P28+P18+M14.1 P28+P19+M14.1 P28+P20+M14.1 P28+P2+M14.1 P1+P18+M14.1 P1+P19+M14.1 P1+P20+M14.1 P1+P2+M14.1

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/18_July"