Team:EPF-Lausanne/Protocols/TetR
From 2011.igem.org
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== Materials == | == Materials == | ||
- | * | + | * 10µl 5x iProof HF buffer |
- | + | *0.5 µl of each primer | |
+ | *1 µl dNTP mix of 10mM | ||
+ | *0.5 µl High Fidelity Polymerase | ||
+ | *1 µl DNA template | ||
+ | *36.5 µl H2O | ||
== Protocol == | == Protocol == |
Revision as of 12:58, 20 June 2011
Linear template- TetR
Materials
- 10µl 5x iProof HF buffer
- 0.5 µl of each primer
- 1 µl dNTP mix of 10mM
- 0.5 µl High Fidelity Polymerase
- 1 µl DNA template
- 36.5 µl H2O
Protocol
- x.
- x