Team:Freiburg/Notebook/4 September

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/3_September">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 4 September </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/5_September">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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===NAME OF YOUR EXPERIMENT===
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===transformation===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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competent E.coli were transformed with 5mikroiter Ligation reaction from [https://2011.igem.org/Team:Freiburg/Notebook/3_September 3 September]
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
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===NAME OF YOUR EXPERIMENT===
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===picking clones===
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'''Investigators:NAME'''
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==<span style="color:red;">red light receptor</span>==
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===NAME OF YOUR EXPERIMENT===
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'''Investigators:NAME'''
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'''Investigators:Sophie'''  <br/>
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No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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<br>
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Another miniprep of the parts was done
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*S66 = modified Lysis genes K124017 in pSB1A2
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*GFP = E0040 in pSB1A2
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*S48 = Quickchange-modified K098995 in pSB1A2
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*S39 = Promotor (J23104) + RBS (B0034) in pSB1C3
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<br>
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As we thought, there is no band for S66, although there is DNA isolated from the mini-prep. It is probably a matter of making a stock from the false mini-prep. More answers on Monday or Tuesday
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<br>
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<br/>
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[[File:9_6_2011_Minis_von_S66_GFP_S48_S39.Jpg‎]]
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<br>
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<br/>
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==

Latest revision as of 00:27, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

transformation

Investigators:Theo

competent E.coli were transformed with 5mikroiter Ligation reaction from 3 September

blue light receptor

picking clones

Investigators:Sophie
No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones

Lysis cassette

3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017

Investigators:Theo
Another miniprep of the parts was done

  • S66 = modified Lysis genes K124017 in pSB1A2
  • GFP = E0040 in pSB1A2
  • S48 = Quickchange-modified K098995 in pSB1A2
  • S39 = Promotor (J23104) + RBS (B0034) in pSB1C3


As we thought, there is no band for S66, although there is DNA isolated from the mini-prep. It is probably a matter of making a stock from the false mini-prep. More answers on Monday or Tuesday



9 6 2011 Minis von S66 GFP S48 S39.Jpg

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/4_September"