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Team:Freiburg/Notebook/4 September
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| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/3_September">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 4 September </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/5_September">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
| - | === | + | ===transformation=== |
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| + | '''Investigators:Theo''' | ||
| + | competent E.coli were transformed with 5mikroiter Ligation reaction from [https://2011.igem.org/Team:Freiburg/Notebook/3_September 3 September] | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
| - | === | + | ===picking clones=== |
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| + | '''Investigators:Sophie''' <br/> | ||
| + | No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones | ||
==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
| - | === | + | ===3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017=== |
'''Investigators:Theo''' | '''Investigators:Theo''' | ||
| + | <br> | ||
| + | Another miniprep of the parts was done | ||
| + | *S66 = modified Lysis genes K124017 in pSB1A2 | ||
| + | *GFP = E0040 in pSB1A2 | ||
| + | *S48 = Quickchange-modified K098995 in pSB1A2 | ||
| + | *S39 = Promotor (J23104) + RBS (B0034) in pSB1C3 | ||
| + | <br> | ||
| + | As we thought, there is no band for S66, although there is DNA isolated from the mini-prep. It is probably a matter of making a stock from the false mini-prep. More answers on Monday or Tuesday | ||
<br> | <br> | ||
| - | + | <br/> | |
| - | File:9_6_2011_Minis_von_S66_GFP_S48_S39.Jpg | + | [[File:9_6_2011_Minis_von_S66_GFP_S48_S39.Jpg]] |
| + | <br> | ||
| + | <br/> | ||
==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== | ||
Latest revision as of 00:27, 22 September 2011
Contact 
This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
green light receptor
transformation
Investigators:Theo
competent E.coli were transformed with 5mikroiter Ligation reaction from 3 September
blue light receptor
picking clones
Investigators:Sophie
No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones
Lysis cassette
3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017
Investigators:Theo
Another miniprep of the parts was done
- S66 = modified Lysis genes K124017 in pSB1A2
- GFP = E0040 in pSB1A2
- S48 = Quickchange-modified K098995 in pSB1A2
- S39 = Promotor (J23104) + RBS (B0034) in pSB1C3
As we thought, there is no band for S66, although there is DNA isolated from the mini-prep. It is probably a matter of making a stock from the false mini-prep. More answers on Monday or Tuesday
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
