"
Team:Groningen/project characterisation mu lasr
From 2011.igem.org
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{HeaderGroningen2011}} | {{HeaderGroningen2011}} | ||
| - | =LasR | + | =LasR autoinducing loop family= |
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM. | Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM. | ||
==Leakage of the LasR autoinducing loops== | ==Leakage of the LasR autoinducing loops== | ||
| - | To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying the | + | To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying an pSB1K3 plasmid with the autoinducing loop biobrick (different variants) and the plasmid pSB1A3 with an input(PLacI controlling LasR) as well as the output (PlasB controlling RFP-LVA). The measurements were done in a presence of 100nM PAI. |
{| class="wikitable" | {| class="wikitable" | ||
Latest revision as of 23:35, 21 September 2011
LasR autoinducing loop family
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
Leakage of the LasR autoinducing loops
To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying an pSB1K3 plasmid with the autoinducing loop biobrick (different variants) and the plasmid pSB1A3 with an input(PLacI controlling LasR) as well as the output (PlasB controlling RFP-LVA). The measurements were done in a presence of 100nM PAI.
| no-tag | DAS | LVA | ||||
| RBS 0,07 |
|
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023] | 
| ||
| RBS 0,3 |
|
|
| |||
| RBS 0,6 |
|
|
| |||
| RBS 1 |
|
|
| |||
| Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL. | ||||||
