Team:Glasgow/Results/MCS
From 2011.igem.org
(Difference between revisions)
HannahRalph (Talk | contribs) |
HannahRalph (Talk | contribs) |
||
Line 11: | Line 11: | ||
<br/><Br/> | <br/><Br/> | ||
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | ||
- | + | <table border="1"> | |
- | < | + | <tr> |
- | + | <td>Forward Primer</td> | |
+ | <td> | ||
5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca | 5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca | ||
agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt | agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt | ||
- | ttttactagtagcggccgctgca-3' | + | ttttactagtagcggccgctgca-3'</td> |
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Reverse Primer</td> | ||
+ | <td>5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG | ||
- | + | TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT | |
- | < | + | CTAGAAGCGGCCGCG-3'</td> |
+ | </tr> | ||
+ | </table> | ||
- | |||
- | |||
- | |||
- | + | <h2>Results</h2> |
Revision as of 22:04, 21 September 2011
![](http://farm7.static.flickr.com/6122/5958857609_b9df31d718_b.jpg)
Aims
- To produce an easier method for testing large numbers of biobricks- To reduce the overall number of ligations needed to do this
Method
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer | 5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt ttttactagtagcggccgctgca-3' |
Reverse Primer | 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT CTAGAAGCGGCCGCG-3' |