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Team:EPF-Lausanne/Our Project/Reporter Systems
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Our overall strategy to develop new transcription factors includes an ''in vivo'' characterization of the affinity and specificity of our TetR mutants. To that end, we set up two reporter systems based on RFP fluorescence. | Our overall strategy to develop new transcription factors includes an ''in vivo'' characterization of the affinity and specificity of our TetR mutants. To that end, we set up two reporter systems based on RFP fluorescence. | ||
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# [[Team:EPF-Lausanne/Our_Project/Assembly/Plac|Plac promoter characterization]] | # [[Team:EPF-Lausanne/Our_Project/Assembly/Plac|Plac promoter characterization]] | ||
# [[Team:EPF-Lausanne/Our_Project/Assembly/Assembly_details|Plasmids details]] | # [[Team:EPF-Lausanne/Our_Project/Assembly/Assembly_details|Plasmids details]] | ||
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Revision as of 22:04, 21 September 2011
In Vivo Characterization
In vivo Main | TetR-RFP System | TetR-LacI-RFP System | Ptet Characterization | Plac Characterization | Plasmid Details
Our overall strategy to develop new transcription factors includes an in vivo characterization of the affinity and specificity of our TetR mutants. To that end, we set up two reporter systems based on RFP fluorescence.
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