Team:Paris Bettencourt/Experiments/tRNA diffusion

From 2011.igem.org

(Difference between revisions)

Revision as of 21:59, 21 September 2011

Team IGEM Paris 2011

Results

We managed to characterize the tRNA biobrick by taking pictures of two constructs described in the following.

GFP amber

One of our strains contains only GFP amber, without the tRNA corresponding. In this situation cells do not glow. This obervation constitutes a reliable negative control.

GFP amber with tRNA amber

The second strain contains the full construct and the production of tRNA is tuned by PHyperspank which is activated by IPTG. In order to make a negative control we plated them without inducing with IPTG. In this situation cells glows with a lower intensity than with the previous construct. We can also notice that some of the cells strongly leak.

GFP amber with tRNA amber induced by IPTG

The last result is given by testing the full construct with IPTG induced cells. Each cell glows with a very weak variability and the luminance is significant.

Characterization of tRNA biobrick

GFP amber / E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)

GFP amber+tRNA amber-IPTG/ E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)

GFP amber + tRNA amber + IPTG/ E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)

@@ Line 3: / Line 3: @@
 ==Results==
-We managed to Characterize the tRNA biobrick by taking pictures of two constructs.
+We managed to characterize the tRNA biobrick by taking pictures of two constructs described in the following.
 ===GFP amber===
-One of our strains contains only GFP amber, without the tTRNA corresponding. Cells do not glow. this result is a reliable negative control.
-===GFP amber+tRNA amber===
+One of our strains contains only GFP amber, without the tRNA corresponding. In this situation cells do not glow. This obervation constitutes a reliable negative control.
-The second strain contains the full construct and the production of tRNA is ruled by PHyperspank which is activated by IPTG.
-In order to make a negative control we plated them without iptg. Cells glows a bit. Moreover some of the cells strongly leak.
+===GFP amber with tRNA amber===
+The second strain contains the full construct and the production of tRNA is tuned by PHyperspank which is activated by IPTG. In order to make a negative control we plated them without inducing with IPTG. In this situation cells glows with a lower intensity than with the previous construct. We can also notice that some of the cells strongly leak.
-===GFP amber + tRNA amber + IPTG===
+===GFP amber with tRNA amber induced by IPTG===
-The last result is given by testing the full construct with IPTG induced cells. Each cell glows in an almost equivalent way. The luminance is significant.
+The last result is given by testing the full construct with IPTG induced cells. Each cell glows with a very weak variability and the luminance is significant.