Team:Paris Bettencourt/Experiments/tRNA diffusion

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Revision as of 21:39, 21 September 2011

Team IGEM Paris 2011

Results

We managed to Characterize the tRNA biobrick by taking pictures of two constructs.

GFP amber

One of our strains contains only GFP amber, without the tTRNA corresponding. Cells do not glow. this result is a reliable negative control.

GFP amber+tRNA amber

The second strain contains the full construct and the production of tRNA is ruled by PHyperspank which is activated by IPTG. In order to make a negative control we plated them without iptg. Cells glows a bit. Moreover some of the cells strongly leak.

GFP amber + tRNA amber + IPTG

The last result is given by testing the full construct with IPTG induced cells. Each cell glows in an almost equivalent way. The luminance is significant.

Characterization of tRNA biobrick

GFP amber / E-coli at 37°C
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)

GFP amber+tRNA amber-IPTG/ E-coli at 37°C
File:Trna noiptg gfp amber trans 1.jpg E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)

@@ Line 28: / Line 28: @@
 |[[File:Trnaminus gfp amber fluo 2.jpg|450px|thumb|center|E-Coli  at 37°C  (gfp image)]]
 |}
+{| border="1" class="wikitable" style="text-align: center;"
+|+GFP amber+tRNA amber-IPTG/ E-coli at 37°C
+|-
+|[[File:Trna noiptg gfp amber trans 1.jpg|450px|thumb|center|E-Coli  at 37°C  (trans image)]]
+|[[File:Trna noiptg gfp amber fluo 1.jpg|450px|thumb|center|E-Coli  at 37°C  (gfp image)]]
+|-
+|[[File:Trna noiptg gfp amber trans 2.jpg|450px|thumb|center|E-Coli  at 37°C  (trans image)]]
+|[[File:Trna noiptg gfp amber fluo 2.jpg|450px|thumb|center|E-Coli  at 37°C  (gfp image)]]
+|}