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Team:Valencia/Notebook/Week4
From 2011.igem.org
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<div class=col_center_top><b>WEEK 4</b></div><!-- fin clase col_left_top--> | <div class=col_center_top><b>WEEK 4</b></div><!-- fin clase col_left_top--> | ||
<div class=col_center_center> | <div class=col_center_center> | ||
| - | <b>Hardware</b> | + | <b>Hardware:</b> We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH. |
Finally we find a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!! | Finally we find a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!! | ||
<br><br> | <br><br> | ||
| - | <b>pH-stat</b> | + | <b>pH-stat:</b> The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.</p> |
| + | |||
| + | <p>Supervision of the evolution of pH and cellular concentration in our cultures, who started working well, increasing the number of cells / ml and the absorbance data was taken at 440 nm.</p> | ||
| + | |||
<br><br> | <br><br> | ||
| - | <b>Colicin production</b> | + | <b>Colicin production:</b> |
</div><!-- fin clase col_left_top--> | </div><!-- fin clase col_left_top--> | ||
Revision as of 20:40, 21 September 2011
WEEK 4
Hardware: We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH.
Finally we find a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!!
pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.
Colicin production:
pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.
Supervision of the evolution of pH and cellular concentration in our cultures, who started working well, increasing the number of cells / ml and the absorbance data was taken at 440 nm.
Colicin production:
