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Parameters

Parameter	Description	Value	Unit	Reference
	Concentration of X	NA	molecules per cell	Notation convention
	Delay due to protein X production and maturation	NA	s	Notation convention
	Maximal production rate of pVeg promoter (constitutive)	0.02	molecules.s-1 or pops	Estimated, see the justification
	Maximal production rate of pHyperSpank promoter	0.02	molecules.s-1 or pops	Estimated, see the justification
	Maximal production rate of pT7 promoter	0.02	molecules.s-1 or pops	Estimated, see the justification
	Maximal production rate of pComK promoter	0.049	molecules.s-1 or pops	[3]
	Maximal production rate of pComS promoter	0.057	molecules.s-1 or pops	[3]
	Maximal production rate of pComG promoter	0.02	molecules.s-1 or pops	Estimated, see the justification
	Basal production rate of pComK promoter	0.0028	molecules.s-1 or pops	[3]
	Basal production rate of pComG promoter	0.028	molecules.s-1 or pops	Is roughly one order of magnitude higher than production rate of pComK[4]
	Dissociation constant for IPTG to LacI	1200	molecules per cell	Aberdeen 2009 wiki
	Dissociation constant for LacI to LacO (pLac)	700	molecules per cell	Aberdeen 2009 wiki
	Dissociation constant for T7 RNA polymerase to pT7	10	molecules per cell	We used the classic assumption 1nM=1 molecule per cell and [1]
	Dissociation constant for ComK to pComK	110	molecules per cell	[3]
	Dissociation constant for ComK to pComS	100	molecules per cell	[3]
	ComK concentration for half maximal degradation	500	molecules per cell	[3]
	ComS concentration for half maximal degradation	50	molecules per cell	[3]
	Hill coefficient for LacI/IPTG interaction	2	NA	Aberdeen 2009 wiki
	Hill coefficient for LacI/pHyperSpank interaction	2	NA	Aberdeen 2009 wiki
	Hill coefficient for ComK/pComK and ComK/pComG (positive feedback) interaction	2	NA	[3]
	Hill coefficient for ComK/pComS (negative feedback) interaction	5	NA	[3]
	Translation rate of proteins	0.9	s-1	Estimated, see the justification
	Dilution rate in exponential phase	2.88x10-4	s-1	Calculated with a 40 min generation time. See explanation
	Unrepressed degradation rate of ComK	1.4x10-3	s-1	[3]
	Unrepressed degradation rate of ComS	1.4x10-3	s-1	[3]
	Degradation rate of mRNA	2.88x10-3	s-1	[4]
	Degradation rate of GFP	10-4	s-1	BioNumbers
	Degradation rate of RFP	10-4	s-1	Estimated equal to GFP degradation rate
	Delay due to CFP production and maturation	360	s	Estimated equal to GFP delay (similar molecules)
	Delay due to YFP production and maturation	360	s	Estimated equal to GFP delay (similar molecules)
	Delay due to ComK production and maturation	300	s	Arbitrary
	Delay due to ComS production and maturation	300	s	Arbitrary
	Delay for ComS repression by ComK	714	s	[3]
	Delay due tT7 RNA polymerase production and maturation	300	s	[2]
	Delay due GFP production and maturation	360	s	BioNumbers
	Delay due RFP production and maturation	360	s	Estimated equal to GFP delay (similar molecules)
	Delay due to mRNA production	30	s	BioNumbers with an approximation: all our contructs are around 1-2kb

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