Team:Wageningen UR/Notebook/Proj2/March

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== March - Fungal Track 'n Trace ==
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<div><a name="March8">March 8</a></div>
 
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<span style="color:white; text-align:justify;"> The pathways have been modelled in Tinkercell. Research has to be done to find orthologs in <span style="font-style:italic;">Aspergillus</span> and to make sure the genes we want to use are really present in <span style="font-style:italic;">Aspergillus</span>. A decision has to be made about what kind of selection markers we are going to use. We are looking for an RFP variant which can be functionally expressed in <span style="font-style:italic;">Aspergillus</span>. </span>
 
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<div><a name="March15">March 15</a></div>
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<span style="color:white; text-align:justify;"> We might have a problem: the mechanism might not work, because there is small leakage from the leu3p repressing system which isn’t wanted because we have an autoinducing system. We have to contact our supervisors about it and talk about it how big the problem is. </span>
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<div><a name="March22">March 22</a></div>
 
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<span style="color:white; text-align:justify;"> The list of the genes is almost finished! Next step: making a BioBrick Design. </span>
 
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<div><a name="March29">March 29</a></div>
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<span style="color:white; text-align:justify;"> There was some debate about whether we should work with <span style="font-style:italic;">Aspergillus nidulans</span> or <span style="font-style:italic;">Aspergillus niger</span>. <span style="font-style:italic;">Aspergillus nidulans</span> is decided to be the best choice. </span>
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'''March 8'''
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Research has to be done to find orthologs in ''Aspergillus'' and to make sure the genes we want to use are really present in ''Aspergillus''. A decision has to be made about what kind of selection markers we are going to use. We are looking for an RFP variant which can be functionally expressed in ''Aspergillus''.
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'''March 15'''
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We might have a problem: the mechanism might not work, because there is small leakage from the leu3p repressing system which isn’t wanted because we have an autoinducing system. We have to contact our supervisors about it and talk about it how big the problem is.
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'''March 22'''
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The list of the genes is almost finished! Next step: making a BioBrick Design.  
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'''March 29'''
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There was some debate about whether we should work with ''Aspergillus nidulans'' or ''Aspergillus niger''. ''Aspergillus nidulans'' is decided to be the best choice.
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[https://2011.igem.org/Team:Wageningen_UR/Notebook/Proj2/February Previous Month]
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[https://2011.igem.org/Team:Wageningen_UR/Notebook/Proj2/April Next Month]
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Latest revision as of 20:23, 21 September 2011

Building a Synchronized Oscillatory System





March - Fungal Track 'n Trace



Mar2.png

March 8

Research has to be done to find orthologs in Aspergillus and to make sure the genes we want to use are really present in Aspergillus. A decision has to be made about what kind of selection markers we are going to use. We are looking for an RFP variant which can be functionally expressed in Aspergillus.


March 15

We might have a problem: the mechanism might not work, because there is small leakage from the leu3p repressing system which isn’t wanted because we have an autoinducing system. We have to contact our supervisors about it and talk about it how big the problem is.


March 22

The list of the genes is almost finished! Next step: making a BioBrick Design.


March 29

There was some debate about whether we should work with Aspergillus nidulans or Aspergillus niger. Aspergillus nidulans is decided to be the best choice.


Previous Month

Next Month

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