Team:DTU-Denmark/Background the natural system
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- | == | + | == Regulation of chitobiose uptake in ''E. coli'' == |
The project is inspired by the regulation of chitobiose uptake and metabolism in ''E.coli''<span class="superscript">[[#References|[1]]]</span>. Especially the following three players, which have been indentified independently in several organisms and thus goes by many names: | The project is inspired by the regulation of chitobiose uptake and metabolism in ''E.coli''<span class="superscript">[[#References|[1]]]</span>. Especially the following three players, which have been indentified independently in several organisms and thus goes by many names: |
Revision as of 19:41, 21 September 2011
The chitobiose system
Regulation of chitobiose uptake in E. coli
The project is inspired by the regulation of chitobiose uptake and metabolism in E.coli[1]. Especially the following three players, which have been indentified independently in several organisms and thus goes by many names:
- 1. The mRNA for a chitoporin that transports chitosugars into cells, called chiP (alias ybfM)
- 2. A sRNA which regulates the chitoporin post-transcriptionally, called chiX (alias sroB, micM)
- 3. A trap-sRNA which is transcribed from intergenic region in the chitobiose operon
- (chbBCARG), which we named chiXR for chiX regulator.
The sRNA (chiX) regulates chitoporin (chiP) expression through binding to the Shine Dalgarno sequence on the chiP mRNA inhibiting recruitment of the 30S ribosome and altering RNA stability. When chitobiose is present, trap-sRNA (chiXR) is transcribed and its transcript binds the sRNA (chiX), relieving chitoporin repression.
![](/wiki/images/6/63/Chitobiose_system.png)
References
[1] Overgaard, Martin, Jesper Johansen, Jakob Møller‐Jensen, and Poul Valentin‐Hansen. “Switching off small RNA regulation with trap‐mRNA.” Molecular Microbiology 73, no. 5 (September 2009): 790-800. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2009.06807.x/abstract.