Team:Groningen/Data page

From 2011.igem.org

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| '''Data For Our Favorite New Parts'''
| '''Data For Our Favorite New Parts'''
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607018 Main Page] - '''Prm_cI-DAS_antiPlasB with 1.0RBS, BBa_K607018''': An auto-inducing loop driven by cI-DAS that shows bimodal behavior without induction.
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607018 Main Page] - '''Prm_cI-DAS_antiPlasB with 1.0RBS, BBa_K607018''': An auto-inducing loop driven by cI-DAS that shows bimodal behavior without induction.
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# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607013 Main Page] - '''Prm_cI-LVA_antiPlasB with 1.0RBS, BBa_K607013''': An auto-inducing loop driven by cI-LVA that is turned off when not induced and might be activated by a high concentration of cI protein.
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# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607013 Main Page] - '''Prm_cI-LVA_antiPlasB with 1.0RBS, BBa_K607013''': An auto-inducing loop driven by cI-LVA that is turned off when not induced. This part is a good candidate for a robust bistable switch.
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607022 Main Page] - '''Prm_cI_antiPlasB with 1.0RBS, BBa_K607022''': An auto-inducing loop driven by cI showing bimodal behavior if uninduced.  
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607022 Main Page] - '''Prm_cI_antiPlasB with 1.0RBS, BBa_K607022''': An auto-inducing loop driven by cI showing bimodal behavior if uninduced.  
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| '''Data For Pre-existing Parts'''
| '''Data For Pre-existing Parts'''
# [http://partsregistry.org/Part:BBa_I0500:Experience Experience] - '''pBAD/araC, a promoter induced by arabinose, BBa_I0500''' (Sri Kosuri 2003) We were able to measure promoter activity with various arabinose concentrations over time which has given us insight into the dynamics of the activation of the promoter.  
# [http://partsregistry.org/Part:BBa_I0500:Experience Experience] - '''pBAD/araC, a promoter induced by arabinose, BBa_I0500''' (Sri Kosuri 2003) We were able to measure promoter activity with various arabinose concentrations over time which has given us insight into the dynamics of the activation of the promoter.  
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# [http://partsregistry.org/Part:BBa_I12006:Experience:Experience Experience] - '''RBS, BBa_I12006''' (Caltech 2004): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR
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# [http://partsregistry.org/Part:BBa_I12006:Experience Experience] - '''cI inducible promoter Prm, BBa_I12006''' (Caltech 2004): We induced the Prm promoter indirectly with cI produced by the protein generator [http://partsregistry.org/wiki/index.php/Part:BBa_K607001 BBa_K607001] controlled by pBAD [http://partsregistry.org/Part:BBa_I0500 BBa_I0500] which enabled us to estimate the activation rate of the transcription factor.
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| '''We've Also Characterized the Following Parts'''
| '''We've Also Characterized the Following Parts'''
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# [http://partsregistry.org/Part:BBa_XXXXX Main Page] - '''Air Freshilizor, BBa_XXXXX''': Our mathematical model predicts that the threshold of activation is 10 parts per billion, the concentration of Butanethiol that humans can typically smell
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[http://partsregistry.org/Part:BBa_J45503:Experience Experience] - '''hybB Cold Shock Promoter, BBa_J45503''': We were able to show that this promoter does not work, which is an important result as this part was really popular among the teams.
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{{FooterGroningen2011}}
{{FooterGroningen2011}}

Revision as of 18:57, 21 September 2011


How Our System Works
Scheme of our circuit
Data For Our Favorite New Parts
  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607018 Main Page] - Prm_cI-DAS_antiPlasB with 1.0RBS, BBa_K607018: An auto-inducing loop driven by cI-DAS that shows bimodal behavior without induction.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607013 Main Page] - Prm_cI-LVA_antiPlasB with 1.0RBS, BBa_K607013: An auto-inducing loop driven by cI-LVA that is turned off when not induced. This part is a good candidate for a robust bistable switch.
  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607022 Main Page] - Prm_cI_antiPlasB with 1.0RBS, BBa_K607022: An auto-inducing loop driven by cI showing bimodal behavior if uninduced.
Data For Pre-existing Parts
  1. [http://partsregistry.org/Part:BBa_I0500:Experience Experience] - pBAD/araC, a promoter induced by arabinose, BBa_I0500 (Sri Kosuri 2003) We were able to measure promoter activity with various arabinose concentrations over time which has given us insight into the dynamics of the activation of the promoter.
  2. [http://partsregistry.org/Part:BBa_I12006:Experience Experience] - cI inducible promoter Prm, BBa_I12006 (Caltech 2004): We induced the Prm promoter indirectly with cI produced by the protein generator [http://partsregistry.org/wiki/index.php/Part:BBa_K607001 BBa_K607001] controlled by pBAD [http://partsregistry.org/Part:BBa_I0500 BBa_I0500] which enabled us to estimate the activation rate of the transcription factor.
We've Also Characterized the Following Parts

[http://partsregistry.org/Part:BBa_J45503:Experience Experience] - hybB Cold Shock Promoter, BBa_J45503: We were able to show that this promoter does not work, which is an important result as this part was really popular among the teams.




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