Revision as of 18:32, 21 September 2011

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muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

Why

How

What

TetR variants	Mutagenesis	Promoter	Cterminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry
V36F	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613013
V36FW43S	PCR-induced	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014
E37AW43ST141A	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613015	X	X
P39K	PCR-induced	T7	His-tag	template	ITT tested, worked	X	BBa_K613016	X	X
Y42F	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613017	X	X
Y42FK108E	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613018	X	X
P39QY42M	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613019
P39QY42ML197S	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613020
P39QY42ML52P	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613021
E37AP39K	site directed	T7	His-tag	linear			BBa_K613022	X
E37AP39QY42F	site directed	T7	His-tag	linear			BBa_K613023	X
WT	none	T7	His&GFP	both

@@ Line 20: / Line 20: @@
 |   V36F ||site directed||SP6||eGFP||plasmid||worked well||X||<html><a href="http://partsregistry.org/Part:BBa_K613013">BBa_K613013</a> </html>||||
 |-
-| <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||
+|  V36FW43S ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||<html> <a href="http://partsregistry.org/Part:BBa_K613014"> BBa_K613014</a> </html>||||
 |-
-| <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||X||BBa_K613015||X||X
+|  E37AW43ST141A ||PCR-induced||T7||His-tag||linear ||ITT tested||X||<html> <a href="http://partsregistry.org/Part:BBa_K613015">BBa_K613015</a></html>||X||X
 |-
-| <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced||T7||His-tag||template ||ITT tested, worked||X||BBa_K613016||X||X
+|  P39K ||PCR-induced||T7||His-tag||template ||ITT tested, worked||X||<html> <a href="http://partsregistry.org/Part:BBa_K613016">BBa_K613016</a> </html>||X||X
 |-
-| <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced||T7||His-tag||linear||ITT tested||X||BBa_K613017||X||X
+| Y42F||PCR-induced||T7||His-tag||linear||ITT tested||X||<html> <a href="http://partsregistry.org/Part:BBa_K613017">BBa_K613017</a></html>||X||X
 |-
-| <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613018||X||X
+| Y42FK108E||PCR-induced||T7||His-tag||linear ||ITT tested||||<html> <a href="http://partsregistry.org/Part:BBa_K613018">BBa_K613018</a></html>||X||X
 |-
-| <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||X||BBa_K613019||||
+| P39QY42M ||site directed||SP6||eGFP||plasmid||worked well||X||<html> <a href="http://partsregistry.org/Part:BBa_K613019">BBa_K613019</a> </html>||||
 |-
 | <html> <a href="http://partsregistry.org/Part:BBa_K613020">P39QY42ML197S</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613020||||

Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

From 2011.igem.org

Revision as of 18:32, 21 September 2011

muTetRs

Why

How

What