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Team:Groningen/fluorimetry
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<br> Inoculate 10ml M9 medium+ antibiotics + 1ml 2% casamino acids and grow the cultures overnight at 37°C. | <br> Inoculate 10ml M9 medium+ antibiotics + 1ml 2% casamino acids and grow the cultures overnight at 37°C. | ||
<br> Grow a logfase to an OD600 of 0.4. | <br> Grow a logfase to an OD600 of 0.4. | ||
Revision as of 17:18, 21 September 2011
Fluorimetry
The OD600 and fluorescence of a great number of samples can be measured the same time with the fluorimeter.
We used it for measuring PBADaraC-cI-LVA+PcI-GFP with different concentrations of arabinose as an induction
signal
Fluorimetry protocol
Inoculate 10ml M9 medium+ antibiotics + 1ml 2% casamino acids and grow the cultures overnight at 37°C.
Grow a logfase to an OD600 of 0.4.
Dilute in a way that the OD600 is decreased to a value of 0.004/
Add 300μl of the diluted culture into a well.
The fluorimeter will measure the OD600+ fluorescence every 15 minutes.
