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Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs
From 2011.igem.org
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| <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014|||| | | <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014|||| | ||
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| - | | <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced||T7||His-tag||linear ||ITT tested|| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||X||BBa_K613015||X||X |
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| - | | <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced||T7||His-tag||template ||ITT tested, worked|| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced||T7||His-tag||template ||ITT tested, worked||X||BBa_K613016||X||X |
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| <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced||T7||His-tag||linear||ITT tested||X||BBa_K613017||X||X | | <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced||T7||His-tag||linear||ITT tested||X||BBa_K613017||X||X | ||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613018||X||X | | <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613018||X||X | ||
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| - | | <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed||SP6||eGFP||plasmid||worked well|| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||X||BBa_K613019|||| |
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| <html> <a href="http://partsregistry.org/Part:BBa_K613020">P39QY42ML197S</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| | | <html> <a href="http://partsregistry.org/Part:BBa_K613020">P39QY42ML197S</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| | ||
Revision as of 17:17, 21 September 2011
muTetRs
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhy
How
What
| TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
|---|---|---|---|---|---|---|---|---|---|
| V36F | site directed | SP6 | eGFP | plasmid | worked well | X | BBa_K613013 | ||
| V36FW43S | PCR-induced | T7 | His-tag | linear | (ins C-term), ITT tested | BBa_K613014 | |||
| E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | X | BBa_K613015 | X | X |
| P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | X | BBa_K613016 | X | X |
| Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | X | BBa_K613017 | X | X |
| Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | X | X | |
| P39QY42M | site directed | SP6 | eGFP | plasmid | worked well | X | BBa_K613019 | ||
| P39QY42ML197S | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613020 | |||
| P39QY42ML52P | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613021 | |||
| E37AP39K | site directed | T7 | His-tag | linear | BBa_K613022 | X | |||
| E37AP39QY42F | site directed | T7 | His-tag | linear | BBa_K613023 | X | |||
| WT | none | T7 | His&GFP | both | BBa_K6130ii |
