Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

From 2011.igem.org

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(→What)
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==What==
==What==
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{| {{table}} style="font-size: 75%;"
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{| style="font-size: 75%;"
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| align="center" style="background:#f0f0f0;"|'''TetR variants'''
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!| TetR variants
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| align="center" style="background:#f0f0f0;"|'''Mutagenesis'''
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!| Mutagenesis
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| align="center" style="background:#f0f0f0;"|'''Promoter'''
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!| Promoter
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| align="center" style="background:#f0f0f0;"|'''Cterminal'''
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!| Cterminal
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| align="center" style="background:#f0f0f0;"|'''DNA form'''
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!| DNA form
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| align="center" style="background:#f0f0f0;"|'''Expression'''
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!| Expression
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| align="center" style="background:#f0f0f0;"|'''MITOMI vs 1-off'''
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!| MITOMI vs 1-off
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| align="center" style="background:#f0f0f0;"|'''Biobrick'''
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!| Biobrick
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| align="center" style="background:#f0f0f0;"|'''Sequenced'''
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!| Sequenced
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| align="center" style="background:#f0f0f0;"|'''Sent to registry'''
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!| Sent to registry
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|-
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| ||||||||||||||||||
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|-
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|  <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||v||BBa_K613013||||
|  <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||v||BBa_K613013||||
|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||
| <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014||||
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v
| <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v
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|-
 
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced||T7||His-tag||template ||ITT tested, worked||v||BBa_K613016||v||v
| <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced||T7||His-tag||template ||ITT tested, worked||v||BBa_K613016||v||v
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v
| <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v
|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v
| <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||v||BBa_K613019||||
| <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed||SP6||eGFP||plasmid||worked well||v||BBa_K613019||||
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| <html> <a href="http://partsregistry.org/Part:BBa_K613021">P39QY42ML52P</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613021||||
| <html> <a href="http://partsregistry.org/Part:BBa_K613021">P39QY42ML52P</a></html>||PCR-induced||T7||His-tag||linear ||ITT tested||||BBa_K613021||||
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613022">E37AP39K</a></html>||site directed||T7||His-tag||linear ||||||BBa_K613022||v||
| <html> <a href="http://partsregistry.org/Part:BBa_K613022">E37AP39K</a></html>||site directed||T7||His-tag||linear ||||||BBa_K613022||v||
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613023">E37AP39QY42F</a></html>||site directed||T7||His-tag||linear ||||||BBa_K613023 ||v||
| <html> <a href="http://partsregistry.org/Part:BBa_K613023">E37AP39QY42F</a></html>||site directed||T7||His-tag||linear ||||||BBa_K613023 ||v||
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|-
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| <html> <a href="http://partsregistry.org/Part:BBa_K613023">WT</a></html>||none||T7||His&GFP||both ||||||BBa_K6130ii || ||
| <html> <a href="http://partsregistry.org/Part:BBa_K613023">WT</a></html>||none||T7||His&GFP||both ||||||BBa_K6130ii || ||

Revision as of 17:16, 21 September 2011

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