"
Team:EPF-Lausanne/Our Project/T7 promoter variants/t7prom
From 2011.igem.org
(Difference between revisions)
(Created page with "{{:Team:EPF-Lausanne/Templates/T7lysisHeader|title=DNA Recovery}} === Introduction === === Characterization of Designed Variants === === Characterization of Randomer Variants...") |
|||
| Line 1: | Line 1: | ||
{{:Team:EPF-Lausanne/Templates/T7lysisHeader|title=DNA Recovery}} | {{:Team:EPF-Lausanne/Templates/T7lysisHeader|title=DNA Recovery}} | ||
| + | == T7 Promoter Variants == | ||
=== Introduction === | === Introduction === | ||
| + | |||
| + | We made two families of T7 promoter variants. Each family has six designed mutants and three randomer mutants, and the difference between the two families is the addition of a lac operator sequence downstream of the T7 promoter. These promoter variants were characterized using fluorescence, revealing a wide range of promoter efficiencies. | ||
| + | |||
| + | === List and Sequences of Variants === | ||
| + | |||
| + | |||
| + | |||
=== Characterization of Designed Variants === | === Characterization of Designed Variants === | ||
Revision as of 17:01, 21 September 2011
Lysis Selection System
Lysis selection system Main | Lysis Characterization | DNA Recovery | DNA Selection | T7 Promoter VariantsContents |
T7 Promoter Variants
Introduction
We made two families of T7 promoter variants. Each family has six designed mutants and three randomer mutants, and the difference between the two families is the addition of a lac operator sequence downstream of the T7 promoter. These promoter variants were characterized using fluorescence, revealing a wide range of promoter efficiencies.
List and Sequences of Variants
Characterization of Designed Variants
Characterization of Randomer Variants
