Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs
From 2011.igem.org
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| <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013|||| | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013|||| | ||
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613014"> V36FW43S</a> </html> ||PCR-induced mutagenesis||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014|||| |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613015"> E37AW43ST141A </a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613016"> P39K </a> </html>||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613017">Y42F</a></html>||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613018">Y42FK108E</a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613019">P39QY42M</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019|||| |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613020">P39QY42ML197S</a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613021">P39QY42ML52P</a></html>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021|||| |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613022">E37AP39K</a></html>||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v|| |
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- | | <html> <a href="http://partsregistry.org/Part: | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613023">E37AP39QY42F</a></html>||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|| |
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{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Revision as of 16:49, 21 September 2011
muTetRs
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhy
How
What
TetR variants | Method | Promoter | C-terminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
V36F | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613013 | ||
V36FW43S | PCR-induced mutagenesis | T7 | His-tag | linear | (ins C-term), ITT tested | BBa_K613014 | |||
E37AW43ST141A | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
P39K | PCR-induced mutagenesis | T7 | His-tag | template | ITT tested and worked ok | v | BBa_K613016 | v | v |
Y42F | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
Y42FK108E | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v | |
P39QY42M | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613019 | ||
P39QY42ML197S | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613020 | |||
P39QY42ML52P | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613021 | |||
E37AP39K | site directed mutagenesis | T7 | His-tag | linear | BBa_K613022 | v | |||
E37AP39QY42F | site directed mutagenesis | T7 | His-tag | linear | BBa_K613023 | v |