Team:Paris Liliane Bettencourt/Notebook/2011/07/05/
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== '''''ligation and transformation''''' == | == '''''ligation and transformation''''' == | ||
- | <p style="display:block;"><u>ligation</u> | + | <p style="display:block;"><font color="red">Axel</font> |
+ | </p><p style="display:block;"><u>ligation</u> | ||
</p> | </p> | ||
- | <ul><li>BBa_k143079 in BBa_K090403 (vector) | + | <ul><li>BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL |
- | + | </li><li>BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL | |
- | </li><li>BBa_K145001 - BBa_B0015 | + | |
</li></ul> | </li></ul> | ||
+ | |||
+ | <p style="display:block;"><font color="red">Babak</font> | ||
+ | </p><p style="display:block;"><u>Gel purification</u> | ||
+ | </p> | ||
+ | <ul><li>well 1: ladder 100pb | ||
+ | </li><li>from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL | ||
+ | </li></ul> | ||
+ | |||
+ | <p style="display:block;"><u>Digestion</u> | ||
+ | <ul><li>BBa_K090403 cut by EcoR1 and XbaI | ||
+ | </li><li>BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx | ||
+ | </li><li>BBa_J18913,2428pb, cut by EcoR1 and XbaI | ||
+ | </li><li>BBa_pHyperspank,121pb, cut by Xbal and Ptsx | ||
+ | </li><li>B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI | ||
+ | </li></ul> | ||
+ | |||
+ | ==Mathias & Laura== | ||
+ | |||
+ | After sequencing, we could see the BioBrick the parts registry sent us under the name K143033 was not LacI but a sorbitol so we have to redo the construction the parts LacI+ double terminator. |
Latest revision as of 15:36, 21 September 2011
![](https://static.igem.org/mediawiki/2011/1/19/Tubeornottube.png)
ligation and transformation
Axel
ligation
- BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
- BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL
Babak
Gel purification
- well 1: ladder 100pb
- from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL
Digestion
- BBa_K090403 cut by EcoR1 and XbaI
- BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
- BBa_J18913,2428pb, cut by EcoR1 and XbaI
- BBa_pHyperspank,121pb, cut by Xbal and Ptsx
- B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI