Team:Paris Liliane Bettencourt/Notebook/2011/07/05/

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{{:Team:Paris_Bettencourt/tpl_test}}
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== '''''ligation and transformation''''' ==
== '''''ligation and transformation''''' ==
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<p style="display:block;"><u>ligation</u>
+
<p style="display:block;"><font color="red">Axel</font>
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</p><p style="display:block;"><u>ligation</u>
</p>
</p>
-
<ul><li>BBa_k143079 in BBa_K090403
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<ul><li>BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
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</li><li>BBa_E1010 - BBa_B0015
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</li><li>BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL
-
</li><li>BBa_K145001 - BBa_B0015
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</li></ul>
</li></ul>
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<p style="display:block;"><font color="red">Babak</font>
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</p><p style="display:block;"><u>Gel purification</u>
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</p>
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<ul><li>well 1: ladder 100pb
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</li><li>from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL
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</li></ul>
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<p style="display:block;"><u>Digestion</u>
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<ul><li>BBa_K090403 cut by EcoR1 and XbaI
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</li><li>BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
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</li><li>BBa_J18913,2428pb, cut by EcoR1 and XbaI
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</li><li>BBa_pHyperspank,121pb, cut by Xbal and Ptsx
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</li><li>B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI
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</li></ul>
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==Mathias & Laura==
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After sequencing, we could see the BioBrick the parts registry sent us under the name K143033 was not LacI but a sorbitol so we have to redo the construction the parts LacI+ double terminator.

Latest revision as of 15:36, 21 September 2011

Team IGEM Paris 2011

ligation and transformation

Axel

ligation

  • BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
  • BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL

Babak

Gel purification

  • well 1: ladder 100pb
  • from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL

Digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
  • BBa_J18913,2428pb, cut by EcoR1 and XbaI
  • BBa_pHyperspank,121pb, cut by Xbal and Ptsx
  • B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI

Mathias & Laura

After sequencing, we could see the BioBrick the parts registry sent us under the name K143033 was not LacI but a sorbitol so we have to redo the construction the parts LacI+ double terminator.

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