Team:Paris Liliane Bettencourt/Notebook/2011/07/04/

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== '''''Minipreps, digestion, electrophoresis and purification''''' ==
== '''''Minipreps, digestion, electrophoresis and purification''''' ==
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</p>
</p>
<ul><li>well 2: BBa_E1010 cut by EcoR1 and SpeI
<ul><li>well 2: BBa_E1010 cut by EcoR1 and SpeI
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</li></ul>
 
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<p style="display:block;"><font color="red">Laura</font>
 
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</p><p style="display:block;"><u>Minipreps</u>
 
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</p>
 
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<ul><li>on BBa_E0040 (vector), concentration of DNA=194.9ng/µL
 
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</li><li>on BBa_K606001 (vector pSB1C3), concentration of DNA=197ng/µL
 
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</li><li>on BBa_I712074 (vector pSB1AAK8), concentration of DNA=166.1ng/µL
 
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</li><li>on BBa_K143053 (pVeg+SpoVg), concentration of DNA=116ng/µL
 
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</li><li>on BBa_K228000 (T7 amber mutation), concentration of DNA=387ng/µL
 
</li></ul>
</li></ul>

Latest revision as of 15:26, 21 September 2011

Team IGEM Paris 2011

Minipreps, digestion, electrophoresis and purification

Axel

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
  • on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
  • on BBa_B0015, concentration of DNA=408.1ng/µL
  • on BBa_E1010 concentration of DNA=330.8ng/µL
  • on BBa_K145001, concentration of DNA=572.8ng/µL


digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_K143079 cut by EcoR1 and SpeI
  • BBa_B0015 cut by EcoR1 and XbaI
  • BBa_E1010 cut by EcoR1 and SpeI
  • BBa_K145001 cut by EcoR1 and SpeI


Electrophoresis 040711.jpg

gel electrophoresis at 0.6% of Agarose

  • well 1: ladder 1kb
  • well 2: BBa_B0015 cut by EcoR1 and XbaI
  • well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
  • well 4: BBa_K145001 cut by EcoR1 and SpeI
  • well 5: BBa_K090403 cut by EcoR1 and XbaI
  • well 6: BBa_K143033 cut by EcoR1 and SpeI


gel purification

  • well 1: ladder 1kb
  • from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
  • from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
  • from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
  • from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL

Babak

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=174ng/µL
  • on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
  • on BBa_B0015, concentration of DNA=375ng/µL
  • on BBa_E1010 (RFP) concentration of DNA=50g/µL
  • after Et-OH reconcentration BBa_E1010: 80ng/µL

digestion

  • well 2: BBa_E1010 cut by EcoR1 and SpeI
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