Team:Paris Liliane Bettencourt/Notebook/2011/07/04/

From 2011.igem.org

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(Minipreps, digestion, purification and ligation)
(Minipreps, digestion, electrophoresis and purification)
 
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== '''''Minipreps, digestion, purification and ligation''''' ==
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{{:Team:Paris_Bettencourt/tpl_test}}
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== '''''Minipreps, digestion, electrophoresis and purification''''' ==
<p style="display:block;"><font color="red">Axel</font>
<p style="display:block;"><font color="red">Axel</font>
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<p style="display:block;"><u>digestion</u>
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</p><p style="display:block;"><u>digestion</u>
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<ul><li>BBa_K090403 cut by EcoR1 and XbaI
<ul><li>BBa_K090403 cut by EcoR1 and XbaI
</li><li>BBa_K143079 cut by EcoR1 and SpeI
</li><li>BBa_K143079 cut by EcoR1 and SpeI
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</li><li>BBa_K145001 cut by EcoR1 and SpeI
</li><li>BBa_K145001 cut by EcoR1 and SpeI
</li></ul>
</li></ul>
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[[File:electrophoresis 040711.jpg|thumb]]
[[File:electrophoresis 040711.jpg|thumb]]
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<ul><li>well 1: ladder 1kb
<ul><li>well 1: ladder 1kb
</li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI
</li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI
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</li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI
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</li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
</li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI
</li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI
</li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI
</li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI
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</li></ul>
</li></ul>
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</p><p style="display:block;"><u>gel purification</u>
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<p style="display:block;"><u>gel purification</u>
</p>
</p>
<ul><li>well 1: ladder 1kb
<ul><li>well 1: ladder 1kb
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</li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI
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</li><li>from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
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</li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI
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</li><li>from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
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</li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI
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</li><li>from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
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</li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI
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</li><li>from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL
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</li><li>well 6: BBa_K143033 cut by EcoR1 and SpeI
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</li></ul>
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<p style="display:block;"><font color="red">Babak</font>
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</p><p style="display:block;"><u>Minipreps</u>
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</p>
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<ul><li>on BBa_K090403 (vector), concentration of DNA=174ng/µL
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</li><li>on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
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</li><li>on BBa_B0015, concentration of DNA=375ng/µL
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</li><li>on BBa_E1010 (RFP) concentration of DNA=50g/µL
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</li><li>after Et-OH reconcentration BBa_E1010: 80ng/µL
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</li></ul>
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<p style="display:block;"><u>digestion</u>
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</p>
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<ul><li>well 2: BBa_E1010 cut by EcoR1 and SpeI
</li></ul>
</li></ul>

Latest revision as of 15:26, 21 September 2011

Team IGEM Paris 2011

Minipreps, digestion, electrophoresis and purification

Axel

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
  • on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
  • on BBa_B0015, concentration of DNA=408.1ng/µL
  • on BBa_E1010 concentration of DNA=330.8ng/µL
  • on BBa_K145001, concentration of DNA=572.8ng/µL


digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_K143079 cut by EcoR1 and SpeI
  • BBa_B0015 cut by EcoR1 and XbaI
  • BBa_E1010 cut by EcoR1 and SpeI
  • BBa_K145001 cut by EcoR1 and SpeI


Electrophoresis 040711.jpg

gel electrophoresis at 0.6% of Agarose

  • well 1: ladder 1kb
  • well 2: BBa_B0015 cut by EcoR1 and XbaI
  • well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
  • well 4: BBa_K145001 cut by EcoR1 and SpeI
  • well 5: BBa_K090403 cut by EcoR1 and XbaI
  • well 6: BBa_K143033 cut by EcoR1 and SpeI


gel purification

  • well 1: ladder 1kb
  • from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
  • from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
  • from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
  • from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL

Babak

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=174ng/µL
  • on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
  • on BBa_B0015, concentration of DNA=375ng/µL
  • on BBa_E1010 (RFP) concentration of DNA=50g/µL
  • after Et-OH reconcentration BBa_E1010: 80ng/µL

digestion

  • well 2: BBa_E1010 cut by EcoR1 and SpeI