Team:Paris Liliane Bettencourt/Notebook/2011/07/04/

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Latest revision as of 15:26, 21 September 2011

Team IGEM Paris 2011

Minipreps, digestion, electrophoresis and purification

Axel

Minipreps

on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
on BBa_B0015, concentration of DNA=408.1ng/µL
on BBa_E1010 concentration of DNA=330.8ng/µL
on BBa_K145001, concentration of DNA=572.8ng/µL

digestion

BBa_K090403 cut by EcoR1 and XbaI
BBa_K143079 cut by EcoR1 and SpeI
BBa_B0015 cut by EcoR1 and XbaI
BBa_E1010 cut by EcoR1 and SpeI
BBa_K145001 cut by EcoR1 and SpeI

gel electrophoresis at 0.6% of Agarose

well 1: ladder 1kb
well 2: BBa_B0015 cut by EcoR1 and XbaI
well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
well 4: BBa_K145001 cut by EcoR1 and SpeI
well 5: BBa_K090403 cut by EcoR1 and XbaI
well 6: BBa_K143033 cut by EcoR1 and SpeI

gel purification

well 1: ladder 1kb
from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL

Babak

Minipreps

on BBa_K090403 (vector), concentration of DNA=174ng/µL
on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
on BBa_B0015, concentration of DNA=375ng/µL
on BBa_E1010 (RFP) concentration of DNA=50g/µL
after Et-OH reconcentration BBa_E1010: 80ng/µL

digestion

well 2: BBa_E1010 cut by EcoR1 and SpeI

@@ Line 1: / Line 1: @@
-== '''''Minipreps, digestion, purification and ligation''''' ==
+{{:Team:Paris_Bettencourt/tpl_test}}
+== '''''Minipreps, digestion, electrophoresis and purification''''' ==
 <p style="display:block;"><font color="red">Axel</font>
@@ Line 12: / Line 14: @@
-<br />10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)
+<p style="display:block;"><u>digestion</u>
-</p><p style="display:block;"><u>digestion</u>
 <ul><li>BBa_K090403 cut by EcoR1 and XbaI
 </li><li>BBa_K143079 cut by EcoR1 and SpeI
@@ Line 23: / Line 22: @@
 </li></ul>
+[[File:electrophoresis 040711.jpg|thumb]]
 </p><p style="display:block;"><u>gel electrophoresis at 0.6% of Agarose</u>
 </p>
 <ul><li>well 1: ladder 1kb
 </li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI
-</li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI
+</li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
 </li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI
 </li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI
@@ Line 33: / Line 34: @@
 </li></ul>
-[[File:electrophoresis 040711.jpg|thumb]]
+<p style="display:block;"><u>gel purification</u>
+</p>
+<ul><li>well 1: ladder 1kb
+</li><li>from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
+</li><li>from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
+</li><li>from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
+</li><li>from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL
+</li></ul>
+<p style="display:block;"><font color="red">Babak</font>
+</p><p style="display:block;"><u>Minipreps</u>
+</p>
+<ul><li>on BBa_K090403 (vector), concentration of DNA=174ng/µL
+</li><li>on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
+</li><li>on BBa_B0015, concentration of DNA=375ng/µL
+</li><li>on BBa_E1010 (RFP) concentration of DNA=50g/µL
+</li><li>after Et-OH reconcentration BBa_E1010: 80ng/µL
+</li></ul>
+<p style="display:block;"><u>digestion</u>
+</p>
+<ul><li>well 2: BBa_E1010 cut by EcoR1 and SpeI
+</li></ul>