Team:EPF-Lausanne/Our Project/T7 promoter variants/recovery

From 2011.igem.org

(Difference between revisions)
(Experimental Setup)
(Experimental Setup)
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[[File:broth_noiptg.png|300px|left]]
[[File:broth_noiptg.png|300px|left]]
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We grow two large cultures of cells. One contains cells that will lyse and release plasmids into the supernatant while the other has non-lysing, "normal" cells.
 
[[File:broth_iptg_induction.png|300px|right]]
[[File:broth_iptg_induction.png|300px|right]]
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Adding IPTG to both flasks induces lysis in one set of cells but not in the others.
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We grow two large cultures of cells. One contains cells that will lyse and release plasmids into the supernatant while the other has non-lysing, "normal" cells. Adding IPTG to both flasks induces lysis in one set of cells but not in the others. If you would like to find out more about how IPTG induction experiments work, please click here.
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[[File:iptg_platecount_text.png|300px]]
=== Results ===
=== Results ===

Revision as of 12:57, 21 September 2011