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Team:EPF-Lausanne/Our Project/T7 promoter variants/recovery
From 2011.igem.org
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| + | We grow two large cultures of cells. One contains cells that will lyse and release plasmids into the supernatant while the other has non-lysing, "normal" cells. | ||
[[File:broth_iptg_induction.png|300px|right]] | [[File:broth_iptg_induction.png|300px|right]] | ||
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| + | Adding IPTG to both flasks induces lysis in one set of cells but not in the others. | ||
=== Results === | === Results === | ||
Revision as of 12:55, 21 September 2011
Lysis Selection System
Lysis selection system Main | Lysis Characterization | DNA Recovery | DNA Selection | T7 Promoter Variants
Introduction
We now wanted to find out if cells release their plasmid DNA into the culture supernatant when they lyse and if we can recover this DNA for further analyses.
Experimental Setup
We grow two large cultures of cells. One contains cells that will lyse and release plasmids into the supernatant while the other has non-lysing, "normal" cells.
Adding IPTG to both flasks induces lysis in one set of cells but not in the others.
Results
