Memory Units

cI Autoinducing Loops

The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.

cI autoinducing loop (BBa_K00) after 4 hours of incubation
	Number  % of total Mean fluorescence Visible 50000 100 --- Fluorescent 9180 18,36 11419 Non-Fluorescent 34424 68 5974	Cel 6
Cel 7	Cel 8	Cel 9