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Team:NTNU Trondheim/Biobricks
From 2011.igem.org
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| - | + | =BioBricks taken from kit= | |
| - | =BioBricks | + | |
{|border="1" cellpadding="4" cellspacing="0" style="border:\#c9c9c9 1px solid; margin: 1em 1em 1em 0;" | {|border="1" cellpadding="4" cellspacing="0" style="border:\#c9c9c9 1px solid; margin: 1em 1em 1em 0;" | ||
| - | | align="center" | + | | align="center";"|'''Short''' |
| - | | align="center" | + | | align="center";"|'''BioBrick''' |
| - | | align="center" | + | | align="center";"|'''Part number''' |
| - | | align="center" | + | | align="center";"|'''Plasmid''' |
| - | | align="center" | + | | align="center";"|'''Comment''' |
|- | |- | ||
| P1||rrnB P1 promoter||BBa_K112118||BBb1 entry vector||Contains the rrnB P1 promoter, possibly also junk. Is in BBb format, not BBa, hence not compatible with other parts we are using. Repressible by ppGpp. | | P1||rrnB P1 promoter||BBa_K112118||BBb1 entry vector||Contains the rrnB P1 promoter, possibly also junk. Is in BBb format, not BBa, hence not compatible with other parts we are using. Repressible by ppGpp. | ||
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| lacI||lacI repressor with RBS||BBa_J24679||pSB1A2||RBS added to LacI protein coding region | | lacI||lacI repressor with RBS||BBa_J24679||pSB1A2||RBS added to LacI protein coding region | ||
|- | |- | ||
| - | | TERM||Double terminator||BBa_B0015||pSB1AK3|| | + | | TERM||Double terminator||BBa_B0015||pSB1AK3||Double terminator, wide usage. |
|- | |- | ||
| ||Strong RBS + LacI(LVAtag) + double terminator||BBa_K292006||pSB1A2 ?||This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. | | ||Strong RBS + LacI(LVAtag) + double terminator||BBa_K292006||pSB1A2 ?||This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. | ||
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| pBAD||Inducible pBad/araC promoter||BBa_I0500||pSB2K3||pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. Repressor: AraC apparently acts as the repressor. | | pBAD||Inducible pBad/araC promoter||BBa_I0500||pSB2K3||pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. Repressor: AraC apparently acts as the repressor. | ||
|- | |- | ||
| - | | pBAD2||Alternative pBAD/araC||BBa_K113009||pSB1A2||Says BBa_I0050 doesn | + | | pBAD2||Alternative pBAD/araC||BBa_K113009||pSB1A2||Says BBa_I0050 doesn't work, made new pBAD/AraC |
| + | |- | ||
| + | | GFP2||Alternative GFP without LVA tag||BBa_E0040||pSB1A2|| | ||
| + | |- | ||
| + | | GFP ssra||Alternative GFP with degradation tag||BBa_E0044||pSB1A3|| | ||
| + | |- | ||
| + | | LacZ||Beta Lactamase + RBS and double terminator||BBa_I732019||BBa_I732950|| | ||
|- | |- | ||
|} | |} | ||
Latest revision as of 14:14, 20 September 2011
BioBricks taken from kit
| Short | BioBrick | Part number | Plasmid | Comment |
| P1 | rrnB P1 promoter | BBa_K112118 | BBb1 entry vector | Contains the rrnB P1 promoter, possibly also junk. Is in BBb format, not BBa, hence not compatible with other parts we are using. Repressible by ppGpp. |
| lambdaPR | lambda pR mod | BBa_R0051 | pSB1A2 | Strong promoter, based on lambda pR, repressible by ci, possibly ppGpp |
| RFP | TetR + p(TetR)+RFP | BBa_K092600 | pSB1A2 | RFP inhibited by tetR, RFP produced by neg. Promoter on TetR |
| Lux | TetR + p(TetR)+RFP+luxI | BBa_K092700 | pSB1A2 | Same as BBa_K092600, but with luxI in addition |
| lacP | lac Promoter (lacI regulated, lambda pL hybrid) | BBa_R0011 | pSB1A2 | Strong promoter, will be on in strains without lacI, off in strains that are lacIq and medium in strains that are lacI |
| lacI | lacI repressor from E. coli (+LVA) | BBa_C0012 | pSB1A2 | Coding region for the LacI protein with an LVA degradation tail and without an RBS. The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG |
| mC | mCherry, bacterial with RBS and forward terminator | BBa_J06702 | pSB1A2 | mCherry, bacterial with RBS and forward terminator |
| lacI | lacI repressor with RBS | BBa_J24679 | pSB1A2 | RBS added to LacI protein coding region |
| TERM | Double terminator | BBa_B0015 | pSB1AK3 | Double terminator, wide usage. |
| Strong RBS + LacI(LVAtag) + double terminator | BBa_K292006 | pSB1A2 ? | This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. | |
| GFP | GFP(+LVA) | BBa_K082003 | pSB1AK3 | GFP with degradation LVA tag |
| RBS | Ribosome Binding Site (strong) | BBa_B0034 | pSB1A2 | Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. Brukes i masse forskjellige constructs |
| TERM2 | Double terminator | BBa_B0014 | pSB1AK3 | |
| pBAD | Inducible pBad/araC promoter | BBa_I0500 | pSB2K3 | pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. Repressor: AraC apparently acts as the repressor. |
| pBAD2 | Alternative pBAD/araC | BBa_K113009 | pSB1A2 | Says BBa_I0050 doesn't work, made new pBAD/AraC |
| GFP2 | Alternative GFP without LVA tag | BBa_E0040 | pSB1A2 | |
| GFP ssra | Alternative GFP with degradation tag | BBa_E0044 | pSB1A3 | |
| LacZ | Beta Lactamase + RBS and double terminator | BBa_I732019 | BBa_I732950 |
