Team:Valencia/Prueba

From 2011.igem.org

(Difference between revisions)
 
(23 intermediate revisions not shown)
Line 4: Line 4:
<style type="text/css">
<style type="text/css">
 +
 +
img {
 +
display: block;
 +
margin: auto;
 +
}
.center {
.center {
 +
width:965px;  
width:965px;  
-
height:200px;
+
height:auto;
-
height:200px;
+
margin-left:7px;
-
        margin-left:32.5px;
+
         margin-right:32.5px;
         margin-right:32.5px;
 +
        margin-top:20px;
 +
margin-bottom:30px;
}
}
.col_left{
.col_left{
-
width:100px;
+
width:650px;
 +
        height:auto;
float:left;
float:left;
-
background-color:#333;
+
background-color:transparent;
-
height:200px;
+
}
}
-
.col_center{
+
.col_left_top{
-
width:100px;
+
width:610px;
-
height:200px;
+
background-color:#0087ba;
-
float:left;
+
height:20px;
-
background-color:red;
+
font-family:Verdana, Arial, Helvetica, sans-serif;
 +
font-size:20px;
 +
color:#fff;
 +
padding:20px;
 +
}
 +
 
 +
.col_left_center{
 +
width:610px;
 +
        text-align:justify;
 +
        height: auto;
 +
font-family:Verdana, Arial, Helvetica, sans-serif;
 +
font-size:14px;
 +
color:black;
 +
padding:20px;
 +
}
 +
 
 +
.col_left_bottom{
 +
width:525px;
 +
        text-align:center;
 +
        height: auto;
 +
font-family:Verdana, Arial, Helvetica, sans-serif;
 +
font-size:14px;
 +
color:#fff;
 +
padding:20px;
}
}
.col_right{
.col_right{
-
width:100px;
+
width:290px;
 +
        margin-left:10px;
float:left;
float:left;
-
background-color:#999;
+
background-color:transparent;
-
height:200px;
+
height:auto;
-
}
+
}
 +
 
 +
.col_right_top{
 +
width:250px;
 +
        background-color:#0087ba;
 +
height:20px;
 +
font-family:Verdana, Arial, Helvetica, sans-serif;
 +
font-size:20px;
 +
color:#fff;
 +
padding:20px;
 +
}
 +
 
 +
.col_right_center{
 +
width:200px;
 +
        height: auto;
 +
font-family:Verdana, Arial, Helvetica, sans-serif;
 +
font-size:14px;
 +
color:#666;
 +
padding:20px;
 +
}
 +
 
 +
.col_right_bottom{
 +
width:335px;
 +
        text-align:center;
 +
        height: auto;
 +
font-family:Verdana, Arial, Helvetica, sans-serif;
 +
font-size:14px;
 +
color:#fff;
 +
padding:20px;
 +
}
 +
 
 +
 
 +
 
Line 41: Line 105:
body {
body {
-
background:darkblue url(https://static.igem.org/mediawiki/2011/a/a7/Valencia_1100_700charcoagua.jpg) no-repeat top fixed;
+
background:darkblue url(https://static.igem.org/mediawiki/2011/6/65/Valencia_Aguamitadinferior1500x800.jpg) repeat-x top fixed;
}
}
Line 47: Line 111:
#globalWrapper { background-color: transparent; border: none; margin: 0; padding: 0;}
#globalWrapper { background-color: transparent; border: none; margin: 0; padding: 0;}
#content{
#content{
-
                 background:transparent url("https://static.igem.org/mediawiki/2010/f/f4/Transpa50.png") center fixed repeat-y;
+
                 background:transparent url("https://static.igem.org/mediawiki/2011/f/fd/Valencia_Transpa30a.png") center fixed repeat-y;
                 padding: 0px;
                 padding: 0px;
        margin:0 auto;
        margin:0 auto;
                 border: none;   
                 border: none;   
 +
           
 +
             }
             }
Line 86: Line 152:
#nav a {
#nav a {
-
display: block;
+
display: block;
-
width: 149px;
+
        width: 149px;
 +
        height: 40px;
 +
        font-family:Verdana, Arial, Helvetica, sans-serif;
}
}
Line 98: Line 166:
#nav li ul {
#nav li ul {
position: absolute;
position: absolute;
-
width: 149px;
+
width: 130px;
-
left: -999em;
+
left: -999em;
 +
}
}
Line 144: Line 213:
a.bannerlinks:hover, a.bannertoplinks:hover {
a.bannerlinks:hover, a.bannertoplinks:hover {
-
   background-color: #99CCFF;
+
   background-color:#00B7FA;
   color: white;
   color: white;
   }
   }
.bannerlinks {
.bannerlinks {
-
background-color:#A3FF47 ;
+
background-color:#70DBFF ;
-
border-style: dotted solid;
+
border-style: none;
border-color: white;
border-color: white;
border-width: 1px;
border-width: 1px;
Line 159: Line 228:
.bannertoplinks {
.bannertoplinks {
-
background-color:#6666FF ;
+
background-color:#5579FF ;
-
border-style: dotted;
+
border-style: none;
border-color: white;
border-color: white;
border-width: 1px;
border-width: 1px;
Line 177: Line 246:
padding: 3px 20px 3px 20px;
padding: 3px 20px 3px 20px;
}
}
 +
Line 217: Line 287:
}
}
      
      
-
.tabla {
 
-
background-color:transparent;
 
-
}
 
-
.tabla th {
 
-
width:70%;
 
-
height:50px;
 
-
}
 
-
.rojo {
 
-
background-color:#33CCFF;
 
-
}
 
-
 
-
.amarillo {
 
-
background-color:#FF6633;
 
-
}
 
-
 
-
.azul {
 
-
background-color:#CC33FF;
 
-
}
 
Line 304: Line 356:
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Team"><b>The team</b></a>
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Team"><b>The team</b></a>
                 <ul style="z-index:1">
                 <ul style="z-index:1">
-
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Team">The team</a></li>
+
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Acknowledgements">Acknowledgements & Attributions</a></li>
-
                        <li><a class="bannerlinks"                  href="https://2011.igem.org/Team:Valencia/Universities">Universities</a></li>
+
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Gallery">Gallery</a></li>
-
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Contact">Contact</a></li>
+
                                     </ul>
                                     </ul>
</li>
</li>
Line 313: Line 364:
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Project"><b>The project</b></a>
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Project"><b>The project</b></a>
                 <ul style="z-index:1">
                 <ul style="z-index:1">
-
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project">Hardware</a></li>
+
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Achievements">Achievements</a></li>
-
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project">Molecular  Biology</a></li>
+
                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Hardware">Hardware</a></li>
-
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project">pH</a></li>
+
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project1">Colicin production</a></li>
 +
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project2">pH-stat</a></li>
 +
                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Modeling">Modeling</a></li>
 +
                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Notebook/Calender">Notebook</a></li>
 +
<li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Protocols">Protocols</a></li>
                 </ul>
                 </ul>
Line 321: Line 376:
         <li>
         <li>
-
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Project"><b>The parts</b></a>
+
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Parts"><b>BioBricks</b></a>
                 <ul style="z-index:1">
                 <ul style="z-index:1">
-
                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project">The parts</a></li>
 
                 </ul>
                 </ul>
</li>
</li>
         <li>
         <li>
-
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Safety"><b>Safety</b></a>
+
<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Safety"><b>Human Practices</b></a>
                 <ul style="z-index:1">
                 <ul style="z-index:1">
-
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safety">Biosafety and Biosecurity</a></li>
+
                         <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safetyquestions">Biosafety and Biosecurity</a></li>
-
<li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safety">Deontological code</a></li>
+
<li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safetycode">Deontological code</a></li>
-
 
+
                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Experts">Asking the experts</a></li>
                 </ul>
                 </ul>
 +
</li>
</li>
</ul>
</ul>
</div>
</div>
 +
<br />
<br />
<br />
<br />
 +
<div class=center>
-
<table class="tabla">
+
<div class=col_left>
-
<tr>
+
<div class=col_left_top>Water Colicin Cleaner: disinfected water by E. coli</div><!-- fin clase col_left_top-->
-
  <th class="rojo">Primera entrada</th>
+
<div class=col_left_center>Water Colicin Cleaner is a biological alternative for disinfecting contaminated water using synthetic biology. We kill targeted pathogens by means of antimicrobial peptides in Escherichia coli. As a proof of concept we have chosen to aim for enterobacteria, focusing on E. coli contaminated water, as a proxy of fecal water. The cleaning of E. coli will be possible thanks to the colicin protein. The activity of these proteins will be controlled by a biological pH-stat, using light-driven cyanobacteria to control pH of the media.
-
  <th class="amarillo">Cabereca 2</th>
+
-
  <th class="azul">Cabereca 3</th>
+
-
</tr>
+
-
<tr>
+
-
  <td>Dato 1</td>
+
-
  <td>Dato 2</td>
+
-
  <td>Dato 3 </td>
+
-
</tr>
+
-
<tr>
+
-
  <td>Dato 4</td>
+
-
  <td>Dato 5</td>
+
-
  <td>Dato 6</td>
+
-
</tr>
+
 +
We will deliver a DIY kit as a prototype platform for killing pathogens responsible of diseases such as cholera, diarrhea and typhoid fever, ending with more than 2 million deaths in the last year according to the 2011 WHO Guidelines for Drinking-Water Quality.
-
</table>  
+
W.C. Cleaner is an effective, low-cost device, that will help people that struggle to find pathogen-free drinking water avoid important diseases. </div><!-- fin clase col_left_top-->
 +
<div class=col_left_bottom><img src="https://static.igem.org/mediawiki/2011/8/81/Valencia_logo.jpg"></div><!-- fin clase col_left_top-->
 +
</div><!-- fin clase col_left-->
-
<br />
+
<div class=col_right>
-
<br />
+
<div class=col_right_top>Who we are</div><!-- fin clase col_right_top-->
-
<br />
+
<div class=col_right_center>
-
<br />
+
<img src="https://static.igem.org/mediawiki/2008/b/b7/Upv_logo.jpg"/ width="200"/>
 +
</div><!-- fin clase col_right_center-->
-
<div class=center>
+
<div class=col_right_center>
-
<div class=col_left></div><!-- fin clase col_left-->
+
<img src="https://static.igem.org/mediawiki/igem.org/c/c4/Valencia_UCVlogo2.jpg" width="200" />
-
<div class=col_center></div><!-- fin clase col_left-->
+
</div><!-- fin clase col_right_center-->
-
<div class=col_right></div><!-- fin clase col_left-->
+
-
</div><!-- fin clase center-->
+
-
==prueba==
+
<div class=col_right_center>
-
 
+
<img src="https://static.igem.org/mediawiki/2008/2/2d/Uv_logo.gif" width="200" />
-
<h3>Under this curious title it is hidden the ambitious Project of Valencia iGEM team. In our project, we intend to present an intermediate scenario in the pathway towards the [[:Team:Valencia/Terraforming | terraforming of Mars]] (i. e., modifying the atmosphere and temperature of Mars in order to get the appropriate conditions to make it habitable for Terran living organisms). The idea is that, after preliminary changes devoted to make Mars conditions more suitable for life, it can be colonized by microorganisms that will accelerate some changes to make the planet conditions acceptable for plant life which, then, will be able to generate enough oxygen to eventually allow the colonization by animals, including humans.</h3>  
+
</div><!-- fin clase col_right_center-->
-
 
+
-
 
+
-
[[Image:Valencia_MarsTransition.jpg|690px|]]
+
 +
<div class=col_right_bottom></div><!-- fin clase col_right_bottom-->
 +
</div><!-- fin clase col_right-->
 +
</div><!-- fin clase center-->
-
Obviously, we do not pretend to follow up all the process, but just to focus on the microorganisms’ colonization step, as motors of the atmosphere building and heating, two essential conditions on the Terraformation process.
 
-
A consequence of the low Martian atmospheric density, the global temperature on Mars depends mainly on the energy exchange between the planet surface and the solar radiation. Therefore, one way to increase the temperature of the planet would be the change of the albedo altering the color of the surface and making it darker.
 
-
[[:Team:Valencia/Project | Our proposal]] is that dark yeast cells would retain the arriving solar radiation and heat the surface. But, once the temperature reaches its optimum on the planetary surface, dark cells will no more be necessary and the color production should be switched off. In order to achieve that and taking advantage of Synthetic Biology principles, a [[:Team:Valencia/prion | switch based on prion proteins]] (on mad yeast!) will be used. 
 
-
On the other hand, a big deal with this approach is the resistance of the organism to violent thermal changes of the Martian surface (summer-winter, day-night) which under certain conditions can be in a range of 20ºC and -80ºC. Thus this work will be complemented with the implementation of the expression of a [[:Team:Valencia/lea | LEA (Late Embryogenesis Abundant) “antifreeze” protein]]. These proteins in both plants and animals are associated with tolerance to water stress resulting from desiccation and cold shock.
 
-
Summarizing, we are going to build a engineered yeast resistant to temperature changes and able to produce a dark pigment which will be the responsible of a global temperature increase on Mars.
 
-
=The Valencia IGEM Team 2010=
 
-
[[Image:valencia_logo_loco_200.png|180px|right]]
 
-
We have the 2010 IGEM team: 9 students from both Universitat de València and Universidad Politècnica de València, go to [[:Team:Valencia/Universities|The Universities]]. Most of us have backgrounds in Biology, Biotechnology, Chemistry, Engineering and Computer Sciences. We want to discover the enigmas of Synthetic Biology and now also the ones tha Mars puts on the table!!!! Go to [[:Team:Valencia/Team|The Team]]  page for more info on us. In the [[:Team:Valencia/Notebook/Calender|Notebook]] you can follow up our work in progress!!! and in [[:Team:Valencia/Gallery|Gallery]] you can see a bunch of pictures of the team working.
 
-
<br>
 
-
=News=
 
-
[[Image:valencia_nasa.jpg|right|140px|NASA]]
 
-
*02/11/2011 - Meeting with David Bergner, Deputy Chief of the [http://spacebiosciences.arc.nasa.gov/ Division of Space Biosciences], from [http://www.nasa.gov/centers/ames/home/index.html NASA Ames Research Center]. Discussed synthetic biology application in space bioscience.
 

Latest revision as of 10:38, 20 September 2011



Water Colicin Cleaner: disinfected water by E. coli
Water Colicin Cleaner is a biological alternative for disinfecting contaminated water using synthetic biology. We kill targeted pathogens by means of antimicrobial peptides in Escherichia coli. As a proof of concept we have chosen to aim for enterobacteria, focusing on E. coli contaminated water, as a proxy of fecal water. The cleaning of E. coli will be possible thanks to the colicin protein. The activity of these proteins will be controlled by a biological pH-stat, using light-driven cyanobacteria to control pH of the media. We will deliver a DIY kit as a prototype platform for killing pathogens responsible of diseases such as cholera, diarrhea and typhoid fever, ending with more than 2 million deaths in the last year according to the 2011 WHO Guidelines for Drinking-Water Quality. W.C. Cleaner is an effective, low-cost device, that will help people that struggle to find pathogen-free drinking water avoid important diseases.
Who we are