"
Team:Imperial College London/Sequence
From 2011.igem.org
| (2 intermediate revisions not shown) | |||
| Line 544: | Line 544: | ||
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p> | TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p> | ||
| - | <h2>Dendra2 forward sequencing results</h2> | + | <h2>Dendra2 forward sequencing results after ligation with Ptet and RBS</h2> |
<p> Dendra_forward_premix -- 15..179 of sequence</p> | <p> Dendra_forward_premix -- 15..179 of sequence</p> | ||
| Line 698: | Line 698: | ||
TTCATCGCTCTGGAGTGA</p> | TTCATCGCTCTGGAGTGA</p> | ||
| + | <h2>Sequence of Dendra2 construct. Verified.</h2> | ||
| + | <p> 71.2F_premix -- 14..961 of sequence | ||
| + | ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA | ||
| + | GGATGATTTCTGGAATTCGCGGCCGCTTCTAGATGAACCTCATCAAAGAGGATATGCGCG | ||
| + | TAAAAGTCCACATGGAAGGCAACGTGAATGGTCATGCGTTCGTAATCGAAGGTGAAGGCA | ||
| + | AAGGGAAACCGTACGAAGGTACGCAAACTGCAAACTTGACCGTGAAAGAAGGTGCACCTC | ||
| + | TGCCATTCAGCTACGACATTCTTACCACTGCGGTTCACTATGGCAATCGTGTCTTTACGA | ||
| + | AATATCCGGAAGATATTCCGGACTACTTCAAACAGTCGTTTCCGGAAGGCTATTCATGGG | ||
| + | AGCGTACCATGACCTTTGAGGATAAAGGCATTTGCACAATTCGCAGCGATATTAGCCTGG | ||
| + | AAGGGGACTGCTTCTTTCAGAACGTTCGGTTTAAAGGCACGAATTTTCCTCCGAATGGGC | ||
| + | CGGTTATGCAGAAGAAAACCCTGAAATGGGAACCGTCGACAGAGAAACTGCATGTGCGTG | ||
| + | ATGGACTTCTGGTCGGTAACATCAACATGGCCTTACTGTTGGAAGGTGGAGGCCATTATC | ||
| + | TCTGTGACTTCAAAACCACGTATAAGGCGAAGAAAGTGGTACAGCTGCCAGATGCCCACT | ||
| + | TTGTGGATCATCGCATCGAAATTCTGGGCAATGACTCCGATTACAACAAAGTCAAGCTGT | ||
| + | ACGAGCATGCTGTTGCTCGCTATAGTCCCTTACCCTCTCAAGTGTGGTGATAATACTAGT | ||
| + | AGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTT | ||
| + | AAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTC | ||
| + | GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT</p> | ||
| + | |||
| + | <p> 71.2R_premix -- 16..995 of sequence | ||
| + | GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT | ||
| + | TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC | ||
| + | GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT | ||
| + | CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA | ||
| + | CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC | ||
| + | AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC | ||
| + | CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA | ||
| + | CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC | ||
| + | CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC | ||
| + | GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT | ||
| + | ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG | ||
| + | GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC | ||
| + | CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT | ||
| + | TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGAAGCGGCCGCGAATTCCAGAAATCA | ||
| + | TCCTTAGCGAAAGCTAAGGATTTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATT | ||
| + | TTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGTGGCACTCGAGTTGAT | ||
| + | CGGGCACGTAAGAGGTTCCA</p> | ||
| + | |||
| + | <h2>Ptet-Dendra2 composite sequencing results. Sequencing results were bad probably due to low concentration of DNA being sent off. One possible base pair mutation and 7% of sequence was not covered.</h2> | ||
| + | |||
| + | <p> 72F_premix -- 15..135 of sequence | ||
| + | TAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG | ||
| + | GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATACTGATC | ||
| + | C</p> | ||
| + | |||
| + | <p>72R_premix -- 20..851 of sequence | ||
| + | ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT | ||
| + | TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC | ||
| + | GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT | ||
| + | CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA | ||
| + | CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC | ||
| + | AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC | ||
| + | CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA | ||
| + | CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC | ||
| + | CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC | ||
| + | GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT | ||
| + | ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG | ||
| + | GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC | ||
| + | CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT | ||
| + | TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGTATTGCTCAGCATCTCT</p> | ||
| + | |||
| + | <h2>J23100-sfGFP composite. Sequence verified.</h2> | ||
| + | |||
| + | |||
| + | <p> 73F_premix -- 22..1003 of sequence | ||
| + | AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG | ||
| + | ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG | ||
| + | TGCTAGCTACTAGAGAAATCAAATTAAGGAGGTAAGATAATGCGTAAAGGCGAAGAACTG | ||
| + | TTCACGGGCGTAGTTCCGATTCTGGTCGAGCTGGACGGCGATGTGAACGGTCATAAGTTT | ||
| + | AGCGTTCGCGGTGAAGGTGAGGGCGACGCGACCAACGGCAAACTGACCCTGAAGTTCATC | ||
| + | TGCACCACCGGTAAACTGCCGGTGCCTTGGCCGACCTTGGTGACGACGTTGACGTATGGC | ||
| + | GTGCAGTGTTTTGCGCGTTATCCGGACCACATGAAACAACACGATTTCTTCAAATCTGCG | ||
| + | ATGCCGGAGGGTTACGTCCAGGAGCGTACCATTTCCTTCAAGGATGATGGCTACTACAAA | ||
| + | ACTCGCGCAGAGGTTAAGTTTGAAGGTGACACGCTGGTCAATCGTATCGAATTGAAGGGT | ||
| + | ATCGACTTTAAAGAGGATGGTAACATTCTGGGCCATAAACTGGAGTATAACTTCAACAGC | ||
| + | CATAATGTTTACATTACGGCAGACAAGCAAAAGAACGGCATCAAGGCCAATTTCAAGATT | ||
| + | CGCCACAATGTTGAGGACGGTAGCGTCCAACTGGCCGACCATTACCAGCAGAACACCCCA | ||
| + | ATTGGTGACGGTCCGGTTTTGCTGCCGGATAATCACTATCTGAGCACCCAAAGCGTGCTG | ||
| + | AGCAAAGATCCGAACGAAAAACGTGATCACATGGTCCTGCTGGAATTTGTGACCGCTGCG | ||
| + | GGCATCACCCACGGTATGGACGAGCTGTATAAGTAATGATACTAGTAGCGGCCGCTGCAG | ||
| + | TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATT | ||
| + | ACTTCGCGTTATGCAGGCTTCC</p> | ||
| + | |||
| + | <p> 73R_premix -- 19..338 of sequence | ||
| + | GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGG | ||
| + | TTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCC | ||
| + | GCTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCA | ||
| + | CAAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTT | ||
| + | GGGTGCTCAGATAGTGATTATCCGGCAGCAAAACCGGACCGTCACCAATTGGGGTGTTCT | ||
| + | GCTGGTAATGGTCGGGTTAC</p> | ||
</html> | </html> | ||
Latest revision as of 09:52, 20 September 2011
Sequencing Results
Vector 7 forward sequencing result
BBa_K316001 (7)_premix -- 19..973 of sequence
TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCA CCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCG CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTC CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGGTAACAGGATTAG CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA
Vector 8 forward sequencing result
BBa_K316005 (8)_premix -- 17..635 of sequence
TAGAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTTACTAGAGCTCATGAACTATGAAGAGGTGACGTCATGAACAAAGGTGTAA TGCGACCGGGCCATGTGCAGCTGCGTGTACTGGACATGAGCAAGGCCCTGGAACACTACG TCGAGTTGCTGGGCCTGATCGAGATGGACCGTGACGACCAGGGCCGTGTCTATCTGAAGG CTTGGACCGAAGTGGATAAGTTTTCCCTGGTGCTACGCGAGGCTGACGAGCCGGGCATGG ATTTTATGGGTTTCAAGGTTGTGGATGAGGATGCTCTCCGGCAACTGGAGCGGGATCTGA TGGCATATGGCTGTGCCGTTGAGCAGCTACCCGCAGGTGAACTGAACAGTTGTGGCCGGC GCGTGCGCTTCCAGGCCCCCTCCGGGCATCACTTCGAGTTGTATGCAGACAAGGAATATA CTGGAAAGTGGGGTTTGAA
Vector 6 forward sequencing result
BBa_K398500 (6)_premix -- 17..960 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCTTCTAGAGAAAGAGGGGACAAACTAGATGCGTAAAGGAGAAGAACTTTTC ACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGC ACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTT CAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATG CCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACA CGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACAC AATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGA CACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCG AAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG ATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT
?
PSVi34-1_premix -- 19..951 of sequence
CATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCT CAGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAA TTACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTAT TCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTT CTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTT GTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTG TTCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCA TGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGA CCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTA TTGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTC ACAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTA GACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAA TTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTAT CCAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTG GTATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCT AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG
?
PSVi34-2_premix -- 21..949 of sequence
ATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCTC AGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAAT TACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTATT CACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTC TGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTTG TACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTGT TCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCAT GCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGAC CAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTAT TGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCA CAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTAG ACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAAT TGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTATC CAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGG TATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCTA GCTAAGATCCGCTCTAACCGAAAAGGAAG
Sequence results for J23113 ligation attempt into RFP. Failed.
52.E_premix -- 14..957 of sequence
ATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCC TCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAAC GGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACC GCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCG CAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTG AAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGT GTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAA CTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGG GAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATG CGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCT AAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCC CACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACC GGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAA TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT
Sequence results for possible auxin producing colony
53_AUX1 -- 13..914 of sequence
GCATCTGATTTTCCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATG AGCACCAGCATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACG TGCCTGAACGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTG CCCCGCGCCCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAG ATAATTGGGGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGC TTACAACGCAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGG ATCGCACGCGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGA AGGACAAAGATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAA CGCGCGGCACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTT ACTCATGGATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAAT TAGCTTTAGATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTG GTGACCCGATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCG CGTTACCGGGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGACAT GCCGTCAGAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGGACACCGGCATG GGTTGAGGGAGCAGTACAAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGG AG
53_AUX2 -- 16..895 of sequence
GGACAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAAC CCACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGG GGACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATG ATTACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTG CGCGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAA GGCGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGG GGGGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGT ACCCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAA GCATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACG AGCATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGA GGTTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGG ACGGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGC CCTACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTG TGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGA AAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG
53_AUX3 -- 15..793 of sequence
GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA
53_premix -- 17..119 of sequence
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTG
53_premix -- 15..843 of sequence
ATATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATATAT GTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTTGG GTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCAGG CTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCCTC CAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCGGA ACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAACT GGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTGAT TGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTCCA AGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATTCA ACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGGAACTGTGGACCAAACTCGTGC CTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGC
Sequence results for possible auxin producing colony
55_AUX1 -- 18..841 of sequence
ATTTTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAG CATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAA CGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGC CCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGG GGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACG CAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACG CGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAA GATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGC ACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGG ATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTA GATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCG ATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCG GGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCA GAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA
55_AUX2 -- 16..893 of sequence
CAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCCA CGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGGA CTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGATT ACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCGC GAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGGC GACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGGG GTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTACC CGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGCA TTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAGC ATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGGT TCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGACG GACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCCT ACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG
55_AUX3 -- 13..851 of sequence
GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGC
55_premix -- 16..869 of sequence
ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAAA CGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGACC GACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGGC CTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCGT CGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCAG CAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCAG ATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGAT GCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACAG CGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAAC AAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTTC GCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGATT AAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAGC TGTGCGCGTCAGAG
55_premix -- 14..888 of sequence
CTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATA TATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACT TGGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGC AGGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGC CTCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGC GGAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAA ACTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGT GATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTT CCAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATAT TCAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGT GCCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAA ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT
Sequence results for our Auxin Xpress module.
56_AUX1 -- 21..841 of sequence
TTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAGCAT CTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAACGC TGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGCCCA GGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGGGGC GATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACGCAG ATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACGCGT TACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAAGAT CCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGCACA TACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGGATG AGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTAGAT GCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCGATT ACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCGGGC CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA
56_AUX2 -- 16..912 of sequence
GCAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCC ACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGG ACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGAT TACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCG CGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGG CGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGG GGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTAC CCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGC ATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAG CATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGG TTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGAC GGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCC TACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGCCGGTGAG
56_AUX3 -- 15..922 of sequence
GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCACGC TTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCA CTCAACGC
56_premix -- 14..930 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGA CAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTG TACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATAT ATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTT GGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCA GGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCC TCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCG GAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAA CTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTG ATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTC CAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATT CAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGTG CCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAAA TTAAGCTTTTACCATCGTGAGGTTTTCGGACAAGTTGGTTTTGGTACAGGGGGTGGGATT CTGATTTTCCAATAGTA
56_premix -- 15..893 of sequence
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAA ACGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGAC CGACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGG CCTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCG TCGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCA GCAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCA GATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGA TGCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACA GCGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAA CAAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTT CGCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGAT TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC
Sequence results to determine SpeI site problem in sfGFP
17R_premix -- 35..933 of sequence
AGCGAGTCGGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAA AAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGGT AGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGCTCTAGTATCATTACTTATACAGCT CGTCCATACCGTGGGTGATGCCCGCAGCGGTCACAAATTCCAACAGGACCATGTGATCAC GTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTGGGTGCTCAGATAGTGATTATCCGGCA GCAAAACCGGACCGTCACCAATTGGGGTGTTCTGCTGGTAATGGTCGGCCAGTTGGACGC TACCGTCCTCAACATTGTGGCGAATCTTGAAATTGGCCTTGATGCCGTTCTTTTGCTTGT CTGCCGTAATGTAAACATTATGGCTGTTGAAGTTATACTCCAGTTTATGGCCCAGAATGT TACCATCCTCTTTAAAGTCGATACCCTTCAATTCGATACGATTGACCAGCGTGTCACCTT CAAACTTAACCTCTGCGCGAGTTTTGTAGTAGCCATCATCCTTGAAGGAAATGGTACGCT CCTGGACGTAACCCTCCGGCATCGCAGATTTGAAGAAATCGTGTTGTTTCATGTGGTCCG GATAACGCGCAAAACACTGCACGCCATACGTCAACGTCGTCACCAAGGTCGGCCAAGGCA CCGGCAGTTTACCGGTGGTGCAGATGAACTTCAGGGTCAGTTTGCCGTTGGTCGCGTCGC CCTCACCTTCACCGCGAACGCTAAACTTATGACCGTTCACATCGCCGTCCAGCTCGACCA GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA
Sequence results for possible PA2652 producing colony
57.20F_premix -- 14..986 of sequence
CTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAG
57.20R_premix -- 13..1023 of sequence
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG
Sequence results for possible PA2652 producing colony
57.3F_premix -- 15..985 of sequence
TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGT ACAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGAC CCTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACT GTCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCG CGAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGG CAGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGC CATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTC TCAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGA TCGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGG TTCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAA ACTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTT GGACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCAC CCTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGG GTTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATG ATATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAAC TCGGTGAACTG
57.3R_premix -- 15..1019 of sequence
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT
Sequence results for possible PA2652 producing colony
57.7F_premix -- 14..987 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAGC
57.7R_premix -- 15..1032 of sequence
GACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTT CGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCG GCCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCA GCTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAA CCGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTT CTGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCG CATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGC TGCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCT GGATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGA CGGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCA GCAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGA CATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCAT GAATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTT GCTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCA TCTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTT CAGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCC CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC
Sequence results for our PA2652 producing construct. Correct.
57.8F_premix -- 15..979 of sequence
TATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACCC TGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTGT CAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGCG AGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGCA GTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCCA TTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCTC AGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGATC GTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGTT CCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAAC TGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTGG ACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACCC TGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGGT TAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGACTCG GTGAC
57.8R_premix -- 14..1022 of sequence
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG
Dendra2 forward sequencing results after ligation with Ptet and RBS
Dendra_forward_premix -- 15..179 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA
Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange
21+60a_frwrd_premix -- 15..921 of sequence
ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG CTGCAGG
Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange
21+60b_frwrd_premix -- 18..928 of sequence
TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT GCAGGCTTCCT
Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange
21+60c_frwrd_premix -- 18..926 of sequence
AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC AGGCTTCCT
Results of corrected SpeI site of sfGFP
sfGFP_reverse_premix -- 20..288 of sequence
ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG GGTGCTCAGATAGTGATTATGCCGGAGCC
Sequence of Anti-holin colony 2
70.2 F_premix -- 25..943 of sequence
AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAGCAACA GCACTTTTTGCCATGCTAGGATTGTCATTTGTTTTATCTCCATCGATTGAAGCGAATGTC GATCCTCATTTTGATAAATTTATGGAATCTGGTATTAGGCACGTTTATATGCTTTTTGAA AATAAAAGCGTAGAATCGTCTGAACAATTCTATAGTTTTATGAGAACGACCTATAAAAAT GACCCGTGCTCTTCTGATTTTGAATGTATAGAGCGAGGCGCGGAGATGGCACAATCATAC GCTAGAATTATGAACATTAAATTGGAGACTGAATAATAACCAGGCATCAAATAAAACGAA AGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCT ACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGG CCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACC GAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGT TCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA AAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAA TCGACGCTCAAGTCAGAGT
70.2 R_premix -- 19..1016 of sequence
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGA GAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTC GTTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTA TGATTGTGCCATCTCCGCGCCTCGCTCTATACATTCAAAATCAGAAGAGCACGGGTCATT TTTATAGGTCGTTCTCATAAAACTATAGAATTGTTCAGACGATTCTACGCTTTTATTTTC AAAAAGCATATAAACGTGCCTAATACCAGATTCCATAAATTTATCAAAATGAGGATCGAC ATTCGCTTCAATCGATGGAGATAAAACAAATGACAATCCTAGCATGGCAAAAAGTGCTGT TGCTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGACCGCAGGCTGGCTAGCACTG TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTAT AGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGG CACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTT GAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATAT ACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTT GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT
Sequence of Anti-holin colony 2
70.9 F_premix -- 18..1008 of sequence
ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAACAA CAGCACTTTGGCACAATCATACGCTAGAATTATGAACATTAAATTGGAGACTGAATAATA ACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGT TGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTC TGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCAC CCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCA CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGC
70.9 R_premix -- 21..998 of sequence
ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGC TACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAG AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCG TTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTAT GATTGTGCCAAAGTGCTGTTGTTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGAC CGCAGGCTGGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGC GAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTC GTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGT GGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATC ACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGA GAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTT TGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTAC GGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT TCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCT GGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTT TTCATCGCTCTGGAGTGA
Sequence of Dendra2 construct. Verified.
71.2F_premix -- 14..961 of sequence ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGATGAACCTCATCAAAGAGGATATGCGCG TAAAAGTCCACATGGAAGGCAACGTGAATGGTCATGCGTTCGTAATCGAAGGTGAAGGCA AAGGGAAACCGTACGAAGGTACGCAAACTGCAAACTTGACCGTGAAAGAAGGTGCACCTC TGCCATTCAGCTACGACATTCTTACCACTGCGGTTCACTATGGCAATCGTGTCTTTACGA AATATCCGGAAGATATTCCGGACTACTTCAAACAGTCGTTTCCGGAAGGCTATTCATGGG AGCGTACCATGACCTTTGAGGATAAAGGCATTTGCACAATTCGCAGCGATATTAGCCTGG AAGGGGACTGCTTCTTTCAGAACGTTCGGTTTAAAGGCACGAATTTTCCTCCGAATGGGC CGGTTATGCAGAAGAAAACCCTGAAATGGGAACCGTCGACAGAGAAACTGCATGTGCGTG ATGGACTTCTGGTCGGTAACATCAACATGGCCTTACTGTTGGAAGGTGGAGGCCATTATC TCTGTGACTTCAAAACCACGTATAAGGCGAAGAAAGTGGTACAGCTGCCAGATGCCCACT TTGTGGATCATCGCATCGAAATTCTGGGCAATGACTCCGATTACAACAAAGTCAAGCTGT ACGAGCATGCTGTTGCTCGCTATAGTCCCTTACCCTCTCAAGTGTGGTGATAATACTAGT AGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTT AAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT
71.2R_premix -- 16..995 of sequence GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGAAGCGGCCGCGAATTCCAGAAATCA TCCTTAGCGAAAGCTAAGGATTTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATT TTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGTGGCACTCGAGTTGAT CGGGCACGTAAGAGGTTCCA
Ptet-Dendra2 composite sequencing results. Sequencing results were bad probably due to low concentration of DNA being sent off. One possible base pair mutation and 7% of sequence was not covered.
72F_premix -- 15..135 of sequence TAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATACTGATC C
72R_premix -- 20..851 of sequence ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATCACCACACTTGAGAGGGTAAGGGACTATAGCGAGCAACAGCATGCT CGTACAGCTTGACTTTGTTGTAATCGGAGTCATTGCCCAGAATTTCGATGCGATGATCCA CAAAGTGGGCATCTGGCAGCTGTACCACTTTCTTCGCCTTATACGTGGTTTTGAAGTCAC AGAGATAATGGCCTCCACCTTCCAACAGTAAGGCCATGTTGATGTTACCGACCAGAAGTC CATCACGCACATGCAGTTTCTCTGTCGACGGTTCCCATTTCAGGGTTTTCTTCTGCATAA CCGGCCCATTCGGAGGAAAATTCGTGCCTTTAAACCGAACGTTCTGAAAGAAGCAGTCCC CTTCCAGGCTAATATCGCTGCGAATTGTGCAAATGCCTTTATCCTCAAAGGTCATGGTAC GCTCCCATGAATAGCCTTCCGGAAACGACTGTTTGAAGTAGTCCGGAATATCTTCCGGAT ATTTCGTAAAGACACGATTGCCATAGTGAACCGCAGTGGTAAGAATGTCGTAGCTGAATG GCAGAGGTGCACCTTCTTTCACGGTCAAGTTTGCAGTTTGCGTACCTTCGTACGGTTTCC CTTTGCCTTCACCTTCGATTACGAACGCATGACCATTCACGTTGCCTTCCATGTGGACTT TTACGCGCATATCCTCTTTGATGAGGTTCATCTAGTATTGCTCAGCATCTCT
J23100-sfGFP composite. Sequence verified.
73F_premix -- 22..1003 of sequence AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCTACTAGAGAAATCAAATTAAGGAGGTAAGATAATGCGTAAAGGCGAAGAACTG TTCACGGGCGTAGTTCCGATTCTGGTCGAGCTGGACGGCGATGTGAACGGTCATAAGTTT AGCGTTCGCGGTGAAGGTGAGGGCGACGCGACCAACGGCAAACTGACCCTGAAGTTCATC TGCACCACCGGTAAACTGCCGGTGCCTTGGCCGACCTTGGTGACGACGTTGACGTATGGC GTGCAGTGTTTTGCGCGTTATCCGGACCACATGAAACAACACGATTTCTTCAAATCTGCG ATGCCGGAGGGTTACGTCCAGGAGCGTACCATTTCCTTCAAGGATGATGGCTACTACAAA ACTCGCGCAGAGGTTAAGTTTGAAGGTGACACGCTGGTCAATCGTATCGAATTGAAGGGT ATCGACTTTAAAGAGGATGGTAACATTCTGGGCCATAAACTGGAGTATAACTTCAACAGC CATAATGTTTACATTACGGCAGACAAGCAAAAGAACGGCATCAAGGCCAATTTCAAGATT CGCCACAATGTTGAGGACGGTAGCGTCCAACTGGCCGACCATTACCAGCAGAACACCCCA ATTGGTGACGGTCCGGTTTTGCTGCCGGATAATCACTATCTGAGCACCCAAAGCGTGCTG AGCAAAGATCCGAACGAAAAACGTGATCACATGGTCCTGCTGGAATTTGTGACCGCTGCG GGCATCACCCACGGTATGGACGAGCTGTATAAGTAATGATACTAGTAGCGGCCGCTGCAG TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATT ACTTCGCGTTATGCAGGCTTCC
73R_premix -- 19..338 of sequence GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGG TTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCA CAAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTT GGGTGCTCAGATAGTGATTATCCGGCAGCAAAACCGGACCGTCACCAATTGGGGTGTTCT GCTGGTAATGGTCGGGTTAC
