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Team:Osaka/week8
From 2011.igem.org
(Difference between revisions)
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!Name!!OD600!!UV(mJ/cm^2) | !Name!!OD600!!UV(mJ/cm^2) | ||
|- | |- | ||
| - | |PprI||0.0109||0,0.5,1,2,5 | + | |PprI||0.0109||0 , 0.5 , 1 , 2 , 5 |
|- | |- | ||
|PprA||0.0418||0,0.5,1,2,5 | |PprA||0.0418||0,0.5,1,2,5 | ||
Revision as of 02:21, 20 September 2011
Contents |
September 18(Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:03,105,106,107,108,109,110,111,112
September 19(Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 113(T):105(upstream)+110(downstream)+1-7A(vector)
- 114(T):111(upstream)+112(downstream)+1-7A(vector)
- Transformation of ligation products
- Measurment of cell survival
| Name | OD600 | UV(mJ/cm^2) |
|---|---|---|
| PprI | 0.0109 | 0 , 0.5 , 1 , 2 , 5 |
| PprA | 0.0418 | 0,0.5,1,2,5 |
| PprM | 0.0454 | 0,0.5,1,2,5 |
| RecA | 0.0114 | 0,0.5,1,2,5 |
| empty vector | 0.0515 | 0,0.5,1,2,5 |
| wild type | 0.0546 | 0,0.5,1,2,5 |
