Team:Osaka/week8

From 2011.igem.org

(Difference between revisions)
Line 9: Line 9:
==September 19(Mon)==
==September 19(Mon)==
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
#* ok
 +
# Ligation
 +
#* 113(T):105(upstream)+110(downstream)+1-7A(vector)
 +
#* 114(T):111(upstream)+112(downstream)+1-7A(vector)
 +
# Transformation of ligation products
 +
# Measurment of cell survival
 +
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 +
|+Table 7
 +
!Name!!OD600!!UV(mJ/cm^2)
 +
|-
 +
|PprI||0.0109||-
 +
|-
 +
|PprA||400μl||2h
 +
|-
 +
|PprM||400μl||1h
 +
|-
 +
|RecA||40μl||2h
 +
|-
 +
|empty vector||40μl||1h
 +
|-
 +
|wild type||40μl||1h
 +
|}

Revision as of 02:17, 20 September 2011


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Measurment of cell survival
Table 7
NameOD600UV(mJ/cm^2)
PprI0.0109-
PprA400μl2h
PprM400μl1h
RecA40μl2h
empty vector40μl1h
wild type40μl1h


September 20(Tue)

September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

Back to Notebook

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