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Team:Cornell/Week 16
From 2011.igem.org
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:::- By deleting excessive nucleotides between the ribosome binding site and the ATG start codon in the original sequences | :::- By deleting excessive nucleotides between the ribosome binding site and the ATG start codon in the original sequences | ||
:'''Work Accomplished''' | :'''Work Accomplished''' | ||
| - | ::*[ | + | ::*[6:10pm] Plated the transformed products of VioA, VioB, VioE, and RFP on agar plates treated with ampicillin or carbenicillin (two are on amp; other two are on carb) |
::*Incubating at 37°C in Olin 303 | ::*Incubating at 37°C in Olin 303 | ||
Revision as of 22:14, 19 September 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 18th - September 24th
Sunday, September 18
Monday, September 19
Morning lab work done by: Jim Mathew
- ...
Afternoon-1 lab work done by: Youjin Cho
- ...
Afternoon-2 lab work done by: Charlie Chung
- Background
- The PCR site-directed mutagenesis done earlier today should ideally enhance protein expression of VioA, VioB, VioE, and RFP
- How?
- - By deleting excessive nucleotides between the ribosome binding site and the ATG start codon in the original sequences
- Work Accomplished
- [6:10pm] Plated the transformed products of VioA, VioB, VioE, and RFP on agar plates treated with ampicillin or carbenicillin (two are on amp; other two are on carb)
- Incubating at 37°C in Olin 303
