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Team:Lyon-INSA-ENS/Realisation/Collection
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| + | <a href="https://2011.igem.org/Main_Page" > | ||
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<br/><br/><br/> | <br/><br/><br/> | ||
<table> | <table> | ||
| + | <caption> Plasmids collection </caption> | ||
<tr> | <tr> | ||
<th> Collection reference </th> <th> Description </th> <th> Resistance(s) </th> | <th> Collection reference </th> <th> Description </th> <th> Resistance(s) </th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>pIG1</td> <td>phaCAB</td> <td> | + | <td>pIG1</td> <td>phaCAB</td> <td> - </td> |
</tr> | </tr> | ||
| - | + | <td>pIG2</td> <td>rcn-curli cloned in pUC57 plasmid</td> <td> Amp </td> | |
| - | <td> | + | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG3</td> <td>Pfort (18A: BBa_J23119)</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG4</td> <td>part Bba_K342000 = Pcurli</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG5</td> <td>enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)</td> <td> Amp+K </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG6</td> <td>pUC18</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG7</td> <td>pUC18</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG8</td> <td>Weak RBS (5L: Bba_J61101) </td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG9</td> <td>GFP with LVA tag (2L: BBa_K145015)</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG10</td> <td>RBS-YFP (22M: BBa_I15017) </td> <td> Amp+K </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG11</td> <td>OmpR234 (BBa_K342003)</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG13</td> <td>RBS-GFP-LVA (BBa_K206009)</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG14</td> <td>Strong RBS (2M: BBa_B0034)</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG15</td> <td>NiCoT</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG16</td> <td>18A-OmpR234 (pIG3+pIG11)</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG17</td> <td>Strong RBS-YFP (pIG14+pIG10) (2M24E)</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG18</td> <td>RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)</td> <td> T </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG19</td> <td>RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)</td> <td> K </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG20</td> <td>Prcn-csg extracted in pUC57 ()</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG21</td> <td>Backbone A = pSB1A3</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG22</td> <td>Backbone K = pSB1K3</td> <td> K </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG23</td> <td>Backbone T = pSB1T3</td> <td> T </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG24</td> <td>Backbone C = pSB1C3</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG25</td> <td>Synthetic part cloned in pSB1C3</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG26</td> <td>part Prcn (= rcnA promoter) </td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG27</td> <td>part Prcn (= rcnA promoter)</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG28</td> <td>part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG29</td> <td>part CsgBA</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG30</td> <td>pIG25</td> <td> - </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG31</td> <td>pKK-OmpR234 (p56 collection biofilm)</td> <td> Amp </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG32</td> <td>pPROBE OT'-Prcn-GFP (p157 collection biofilm)</td> <td> Spc </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG33</td> <td>PrcnA-OmpR234</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG34</td> <td>PrcnA-OmpR234</td> <td> K </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>pIG35</td> <td>= pIG25</td> <td> Cm </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | |
| + | </table> | ||
| + | |||
| + | |||
| + | <br/><br/><br/><br/> | ||
| + | |||
| + | <table> | ||
| + | <caption> Strains collection </caption> | ||
| + | <tr> | ||
| + | <th> Collection reference </th> <th> Description </th> <th> Phenotype </th> | ||
</tr> | </tr> | ||
| + | <tr> | ||
| + | <td>S5</td> <td></td> <td> Amp resistant </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S6</td> <td>NM522/pIG26</td> <td> Cm resistant</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S7</td> <td>MC4100</td> <td>streptomycin resistant </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S8</td> <td>MG1655</td> <td> - </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S9</td> <td>NM522/pIG27</td> <td>Cm resistant </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S10</td> <td>NM522/pIG26</td> <td>Cm resistant </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S11</td> <td>NM522</td> <td> - </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S12</td> <td>DH5α</td> <td>nalidixic acid resistant </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S13</td> <td>NMYO</td> <td>Kan resistant </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S14</td> <td>NM522 + pTNiCo</td> <td>Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S15</td> <td>PHL818</td> <td>Tet resistant, sticky</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S16</td> <td>PHL1273</td> <td>Tet resistant, Kan resistant</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S17</td> <td>NM522/pIG25</td> <td>Cm resistant</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S18</td> <td>NM522/pIG2</td> <td>Amp resistant</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S19=S20</td> <td>NM522/pIG16 </td> <td>Amp resistant</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S21</td> <td>NM522/pIG6</td> <td>AmpR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S22</td> <td>NM522/pIG33</td> <td> - </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S23</td> <td>PHL1414/pIG2</td> <td> CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S24</td> <td>PHL1414/pIG6</td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S25</td> <td>MC4100/piG6</td> <td>AmpR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S26</td> <td>MC4100/piG2</td> <td>AmpR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S27</td> <td>MC4100/pIG27</td> <td>CmR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S28</td> <td>MC4100/pIG16</td> <td>AmpR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S29</td> <td>PHL1414</td> <td>CmR (genomic), constitutively fluorescent</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S30</td> <td>PHL1414/pIG3</td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S31</td> <td>PHL1414/pIG16 </td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S32</td> <td>MC4100/pIG34</td> <td>KanR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>S33</td> <td>MC4100/pIG33</td> <td>CmR</td> | ||
| + | </tr> | ||
</table> | </table> | ||
Latest revision as of 17:05, 19 September 2011
| Collection reference | Description | Resistance(s) |
|---|---|---|
| pIG1 | phaCAB | - | pIG2 | rcn-curli cloned in pUC57 plasmid | Amp |
| pIG3 | Pfort (18A: BBa_J23119) | Amp |
| pIG4 | part Bba_K342000 = Pcurli | Cm |
| pIG5 | enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) | Amp+K |
| pIG6 | pUC18 | Amp |
| pIG7 | pUC18 | Amp |
| pIG8 | Weak RBS (5L: Bba_J61101) | Amp |
| pIG9 | GFP with LVA tag (2L: BBa_K145015) | Amp |
| pIG10 | RBS-YFP (22M: BBa_I15017) | Amp+K |
| pIG11 | OmpR234 (BBa_K342003) | Cm |
| pIG13 | RBS-GFP-LVA (BBa_K206009) | Cm |
| pIG14 | Strong RBS (2M: BBa_B0034) | Amp |
| pIG15 | NiCoT | Amp |
| pIG16 | 18A-OmpR234 (pIG3+pIG11) | Amp |
| pIG17 | Strong RBS-YFP (pIG14+pIG10) (2M24E) | Amp |
| pIG18 | RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) | T |
| pIG19 | RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) | K |
| pIG20 | Prcn-csg extracted in pUC57 () | Amp |
| pIG21 | Backbone A = pSB1A3 | Amp |
| pIG22 | Backbone K = pSB1K3 | K |
| pIG23 | Backbone T = pSB1T3 | T |
| pIG24 | Backbone C = pSB1C3 | Cm |
| pIG25 | Synthetic part cloned in pSB1C3 | Cm |
| pIG26 | part Prcn (= rcnA promoter) | Cm |
| pIG27 | part Prcn (= rcnA promoter) | Cm |
| pIG28 | part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) | Cm |
| pIG29 | part CsgBA | Amp |
| pIG30 | pIG25 | - |
| pIG31 | pKK-OmpR234 (p56 collection biofilm) | Amp |
| pIG32 | pPROBE OT'-Prcn-GFP (p157 collection biofilm) | Spc |
| pIG33 | PrcnA-OmpR234 | Cm |
| pIG34 | PrcnA-OmpR234 | K |
| pIG35 | = pIG25 | Cm |
| Collection reference | Description | Phenotype |
|---|---|---|
| S5 | Amp resistant | |
| S6 | NM522/pIG26 | Cm resistant |
| S7 | MC4100 | streptomycin resistant |
| S8 | MG1655 | - |
| S9 | NM522/pIG27 | Cm resistant |
| S10 | NM522/pIG26 | Cm resistant |
| S11 | NM522 | - |
| S12 | DH5α | nalidixic acid resistant |
| S13 | NMYO | Kan resistant |
| S14 | NM522 + pTNiCo | Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19 |
| S15 | PHL818 | Tet resistant, sticky |
| S16 | PHL1273 | Tet resistant, Kan resistant |
| S17 | NM522/pIG25 | Cm resistant |
| S18 | NM522/pIG2 | Amp resistant |
| S19=S20 | NM522/pIG16 | Amp resistant |
| S21 | NM522/pIG6 | AmpR |
| S22 | NM522/pIG33 | - |
| S23 | PHL1414/pIG2 | CmR (genomic), AmpR (plasmidic). Constitutively fluorescent |
| S24 | PHL1414/pIG6 | CmR (genomic), AmpR (plasmidic). Constitutively fluorescent |
| S25 | MC4100/piG6 | AmpR |
| S26 | MC4100/piG2 | AmpR |
| S27 | MC4100/pIG27 | CmR |
| S28 | MC4100/pIG16 | AmpR |
| S29 | PHL1414 | CmR (genomic), constitutively fluorescent |
| S30 | PHL1414/pIG3 | CmR (genomic), AmpR (plasmidic). Constitutively fluorescent |
| S31 | PHL1414/pIG16 | CmR (genomic), AmpR (plasmidic). Constitutively fluorescent |
| S32 | MC4100/pIG34 | KanR |
| S33 | MC4100/pIG33 | CmR |
