Team:Lyon-INSA-ENS/Realisation/Collection

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<br/><br/><br/>
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<table>
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<caption> Plasmids collection </caption>
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<tr>
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<th> Collection reference </th> <th> Description </th> <th> Resistance(s) </th>
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</tr>
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<tr>
 +
<td>pIG1</td> <td>phaCAB</td> <td> - </td>
 +
</tr>
 +
<td>pIG2</td> <td>rcn-curli cloned in pUC57 plasmid</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG3</td> <td>Pfort (18A: BBa_J23119)</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG4</td> <td>part Bba_K342000 = Pcurli</td> <td> Cm </td>
 +
</tr>
 +
<tr>
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<td>pIG5</td> <td>enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030)</td> <td> Amp+K </td>
 +
</tr>
 +
<tr>
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<td>pIG6</td> <td>pUC18</td> <td> Amp </td>
 +
</tr>
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<tr>
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<td>pIG7</td> <td>pUC18</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG8</td> <td>Weak RBS (5L: Bba_J61101) </td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG9</td> <td>GFP with LVA tag (2L: BBa_K145015)</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG10</td> <td>RBS-YFP (22M: BBa_I15017) </td> <td> Amp+K </td>
 +
</tr>
 +
<tr>
 +
<td>pIG11</td> <td>OmpR234 (BBa_K342003)</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG13</td> <td>RBS-GFP-LVA (BBa_K206009)</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG14</td> <td>Strong RBS (2M: BBa_B0034)</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG15</td> <td>NiCoT</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG16</td> <td>18A-OmpR234 (pIG3+pIG11)</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG17</td> <td>Strong RBS-YFP (pIG14+pIG10) (2M24E)</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG18</td> <td>RBSfort-GFP-Tet (pIG14+pIG9) (2M2L)</td> <td> T </td>
 +
</tr>
 +
<tr>
 +
<td>pIG19</td> <td>RBSfort-GFP-Kan (pIG14+pIG9) (2M2L)</td> <td> K </td>
 +
</tr>
 +
<tr>
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<td>pIG20</td> <td>Prcn-csg extracted in pUC57 ()</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG21</td> <td>Backbone A = pSB1A3</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG22</td> <td>Backbone K = pSB1K3</td> <td> K </td>
 +
</tr>
 +
<tr>
 +
<td>pIG23</td> <td>Backbone T = pSB1T3</td> <td> T </td>
 +
</tr>
 +
<tr>
 +
<td>pIG24</td> <td>Backbone C = pSB1C3</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG25</td> <td>Synthetic part cloned in pSB1C3</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG26</td> <td>part Prcn (= rcnA promoter) </td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG27</td> <td>part Prcn (= rcnA promoter)</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG28</td> <td>part Pcurli-RBS-GFP(LVA) (pIG4+pIG18)</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG29</td> <td>part CsgBA</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG30</td> <td>pIG25</td> <td> - </td>
 +
</tr>
 +
<tr>
 +
<td>pIG31</td> <td>pKK-OmpR234 (p56 collection biofilm)</td> <td> Amp </td>
 +
</tr>
 +
<tr>
 +
<td>pIG32</td> <td>pPROBE OT'-Prcn-GFP (p157 collection biofilm)</td> <td> Spc </td>
 +
</tr>
 +
<tr>
 +
<td>pIG33</td> <td>PrcnA-OmpR234</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
<td>pIG34</td> <td>PrcnA-OmpR234</td> <td> K </td>
 +
</tr>
 +
<tr>
 +
<td>pIG35</td> <td>= pIG25</td> <td> Cm </td>
 +
</tr>
 +
<tr>
 +
 
 +
</table>
 +
 
 +
 
 +
<br/><br/><br/><br/>
 +
 
 +
<table>
 +
<caption> Strains collection </caption>
 +
<tr>
 +
<th> Collection reference </th> <th> Description </th> <th> Phenotype </th>
 +
</tr>
 +
<tr>
 +
<td>S5</td> <td></td> <td> Amp resistant </td>
 +
</tr>      
 +
<tr>
 +
<td>S6</td> <td>NM522/pIG26</td> <td> Cm resistant</td>
 +
</tr> 
 +
<tr>
 +
<td>S7</td> <td>MC4100</td> <td>streptomycin resistant </td>
 +
</tr> 
 +
<tr>
 +
<td>S8</td> <td>MG1655</td> <td> - </td>
 +
</tr> 
 +
<tr>
 +
<td>S9</td> <td>NM522/pIG27</td> <td>Cm resistant </td>
 +
</tr> 
 +
<tr>
 +
<td>S10</td> <td>NM522/pIG26</td> <td>Cm resistant </td>
 +
</tr> 
 +
<tr>
 +
<td>S11</td> <td>NM522</td> <td> - </td>
 +
</tr> 
 +
<tr>
 +
<td>S12</td> <td>DH5α</td> <td>nalidixic acid resistant  </td>
 +
</tr> 
 +
<tr>
 +
<td>S13</td> <td>NMYO</td> <td>Kan resistant </td>
 +
</tr> 
 +
<tr>
 +
<td>S14</td> <td>NM522 + pTNiCo</td> <td>Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19</td>
 +
</tr> 
 +
<tr>
 +
<td>S15</td> <td>PHL818</td> <td>Tet resistant, sticky</td>
 +
</tr> 
 +
<tr>
 +
<td>S16</td> <td>PHL1273</td> <td>Tet resistant, Kan resistant</td>
 +
</tr> 
 +
<tr>
 +
<td>S17</td> <td>NM522/pIG25</td> <td>Cm resistant</td>
 +
</tr> 
 +
<tr>
 +
<td>S18</td> <td>NM522/pIG2</td> <td>Amp resistant</td>
 +
</tr> 
 +
<tr>
 +
<td>S19=S20</td> <td>NM522/pIG16 </td> <td>Amp resistant</td>
 +
</tr>    
 +
<tr>
 +
<td>S21</td> <td>NM522/pIG6</td> <td>AmpR</td>
 +
</tr> 
 +
<tr>
 +
<td>S22</td> <td>NM522/pIG33</td> <td> - </td>
 +
</tr> 
 +
<tr>
 +
<td>S23</td> <td>PHL1414/pIG2</td> <td> CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 +
</tr> 
 +
 
 +
<tr>
 +
<td>S24</td> <td>PHL1414/pIG6</td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 +
</tr> 
 +
<tr>
 +
<td>S25</td> <td>MC4100/piG6</td> <td>AmpR</td>
 +
</tr> 
 +
<tr>
 +
<td>S26</td> <td>MC4100/piG2</td> <td>AmpR</td>
 +
</tr> 
 +
<tr>
 +
<td>S27</td> <td>MC4100/pIG27</td> <td>CmR</td>
 +
</tr> 
 +
<tr>
 +
<td>S28</td> <td>MC4100/pIG16</td> <td>AmpR</td>
 +
</tr> 
 +
<tr>
 +
<td>S29</td> <td>PHL1414</td> <td>CmR (genomic), constitutively fluorescent</td>
 +
</tr> 
 +
<tr>
 +
<td>S30</td> <td>PHL1414/pIG3</td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 +
</tr> 
 +
<tr>
 +
<td>S31</td> <td>PHL1414/pIG16 </td> <td>CmR (genomic), AmpR (plasmidic). Constitutively fluorescent</td>
 +
</tr> 
 +
<tr>
 +
<td>S32</td> <td>MC4100/pIG34</td> <td>KanR</td>
 +
</tr> 
 +
<tr>
 +
<td>S33</td> <td>MC4100/pIG33</td> <td>CmR</td>
 +
</tr> 
 +
</table>
</div>
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Latest revision as of 17:05, 19 September 2011






Plasmids collection
Collection reference Description Resistance(s)
pIG1 phaCAB -
pIG2 rcn-curli cloned in pUC57 plasmid Amp
pIG3 Pfort (18A: BBa_J23119) Amp
pIG4 part Bba_K342000 = Pcurli Cm
pIG5 enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) Amp+K
pIG6 pUC18 Amp
pIG7 pUC18 Amp
pIG8 Weak RBS (5L: Bba_J61101) Amp
pIG9 GFP with LVA tag (2L: BBa_K145015) Amp
pIG10 RBS-YFP (22M: BBa_I15017) Amp+K
pIG11 OmpR234 (BBa_K342003) Cm
pIG13 RBS-GFP-LVA (BBa_K206009) Cm
pIG14 Strong RBS (2M: BBa_B0034) Amp
pIG15 NiCoT Amp
pIG16 18A-OmpR234 (pIG3+pIG11) Amp
pIG17 Strong RBS-YFP (pIG14+pIG10) (2M24E) Amp
pIG18 RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) T
pIG19 RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) K
pIG20 Prcn-csg extracted in pUC57 () Amp
pIG21 Backbone A = pSB1A3 Amp
pIG22 Backbone K = pSB1K3 K
pIG23 Backbone T = pSB1T3 T
pIG24 Backbone C = pSB1C3 Cm
pIG25 Synthetic part cloned in pSB1C3 Cm
pIG26 part Prcn (= rcnA promoter) Cm
pIG27 part Prcn (= rcnA promoter) Cm
pIG28 part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) Cm
pIG29 part CsgBA Amp
pIG30 pIG25 -
pIG31 pKK-OmpR234 (p56 collection biofilm) Amp
pIG32 pPROBE OT'-Prcn-GFP (p157 collection biofilm) Spc
pIG33 PrcnA-OmpR234 Cm
pIG34 PrcnA-OmpR234 K
pIG35 = pIG25 Cm




Strains collection
Collection reference Description Phenotype
S5 Amp resistant
S6 NM522/pIG26 Cm resistant
S7 MC4100 streptomycin resistant
S8 MG1655 -
S9 NM522/pIG27 Cm resistant
S10 NM522/pIG26 Cm resistant
S11 NM522 -
S12 DH5α nalidixic acid resistant
S13 NMYO Kan resistant
S14 NM522 + pTNiCo Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19
S15 PHL818 Tet resistant, sticky
S16 PHL1273 Tet resistant, Kan resistant
S17 NM522/pIG25 Cm resistant
S18 NM522/pIG2 Amp resistant
S19=S20 NM522/pIG16 Amp resistant
S21 NM522/pIG6 AmpR
S22 NM522/pIG33 -
S23 PHL1414/pIG2 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S24 PHL1414/pIG6 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S25 MC4100/piG6 AmpR
S26 MC4100/piG2 AmpR
S27 MC4100/pIG27 CmR
S28 MC4100/pIG16 AmpR
S29 PHL1414 CmR (genomic), constitutively fluorescent
S30 PHL1414/pIG3 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S31 PHL1414/pIG16 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S32 MC4100/pIG34 KanR
S33 MC4100/pIG33 CmR





ENS assystem Biomérieux INSA INSA
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