Team:DTU-Denmark/PCR product purification

From 2011.igem.org

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{{:Team:DTU-Denmark/Templates/Standard_page_begin|PCR product purification}}
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== PCR product purification ==
== PCR product purification ==
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# Add 30 µl Elution Buffer NE and incubate at RT for 1 min to increase the yield of eluted DNA.
# Add 30 µl Elution Buffer NE and incubate at RT for 1 min to increase the yield of eluted DNA.
# Centrifuge at 11.000 g for 1 min.
# Centrifuge at 11.000 g for 1 min.
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{{:Team:DTU-Denmark/Templates/Standard_page_end}}

Revision as of 14:48, 18 September 2011

PCR product purification

PCR product purification

  1. Mix 1 volume of sample with 2 volumes of NT buffer in an 1,5 ml Eppendorf tube.
  2. Place a column into a 2 ml collection tube and load the sample,
  3. Centrifuge at 11.000 g for 1 min.
  4. Discard flow through and place the column back into the collection tube.
  5. Add 600 µl NT3 buffer and centrifuge at 11.000 g for 1 min.
  6. Discard flow through and place the column back into the collection tube.
  7. Centrifuge at 11.000 g for 2 min to remove NT3 buffer. Discard flow through.
  8. Place the column into a clean 1,5 ml Eppendorf tube.
  9. Add 30 µl Elution Buffer NE and incubate at RT for 1 min to increase the yield of eluted DNA.
  10. Centrifuge at 11.000 g for 1 min.

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