Latest revision as of 02:40, 16 September 2011

General Ligation Protocol

7uL insert

1uL vector

1uL T4 ligase buffer

1uL T4 ligase

Incubate at 16C overnight, or room temperature for 30 minutes to 1 hour.

Revision as of 01:32, 14 September 2011 (view source) A.uw (Talk \| contribs) m ← Older edit		Latest revision as of 02:40, 16 September 2011 (view source) Mills (Talk \| contribs) (→General Ligation Protocol)
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-	Incubate at ~~<s>37C</s> no. **Note that NEB website recommends~~ 16C - room ~~temp~~ for ~~ligations, we do ours at RT and it works well.~~ 1 hour~~, or less~~.	+	Incubate at 16C overnight, or room temperature for 30 minutes to 1 hour.