Team:Washington/Protocols/Overnights.

From 2011.igem.org

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=Preparation of Overnight Cultures=
=Preparation of Overnight Cultures=
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# Obtain a 14 ml Sterile Polypropylene Round-Bottom Tube.
+
# Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube.
-
# Add ~ 3-5 mls of an appropriate broth (either LB or TB)
+
# Add ~ 3-5 mLs of an appropriate broth (either LB or TB)
-
#
+
# Add an appropriate antibiotic if necessary and set this tube safely aside
 +
#* Amp (1:100)
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#* Chlor (1:1000)
 +
#* Kan (1:1000)
 +
# Prick the desired colony off a plate and suspend it in 50 uL PBS.
 +
#* Refer to the PBS stock making protocol!
 +
# Add 10 uL of the PBS sample and add it to the broth previously set aside.
 +
#* Incubate overnight cultures @ 37oC, overnight with shaking.

Latest revision as of 18:13, 15 September 2011


Preparation of Overnight Cultures

  1. Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube.
  2. Add ~ 3-5 mLs of an appropriate broth (either LB or TB)
  3. Add an appropriate antibiotic if necessary and set this tube safely aside
    • Amp (1:100)
    • Chlor (1:1000)
    • Kan (1:1000)
  4. Prick the desired colony off a plate and suspend it in 50 uL PBS.
    • Refer to the PBS stock making protocol!
  5. Add 10 uL of the PBS sample and add it to the broth previously set aside.
    • Incubate overnight cultures @ 37oC, overnight with shaking.
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