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Team:Osaka/week7
From 2011.igem.org
(Difference between revisions)
(→September 14(Wed)) |
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==September 15(Thu)== | ==September 15(Thu)== | ||
| - | + | # Miniprep of yesterday's culture | |
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | # To liquid culture:108 | ||
==September 16(Fri)== | ==September 16(Fri)== | ||
Revision as of 11:23, 15 September 2011
Contents |
September 11(Sun)
- Gel extraction of digests
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 103(C):PprM+1-3A(vector)
- 104(C):RecA+1-3A(vector)
- 105(A):12(upstream)+101(downstream)+1-1G(vector)
- 106(A):12(upstream)+102(downstream)+1-1G(vector)
- Transformation of ligation products
September 12(Mon)
- To liquid culture:103,104,105,106
September 13(Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 107(A):12(upstream)+103(downstream)+1-1G(vector)
- 108(A):12(upstream)+104(downstream)+1-1G(vector)
- Transformation of ligation products
September 14(Wed)
- To liquid culture:107,108
September 15(Thu)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:108
