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Team:UT-Tokyo/LabNote
From 2011.igem.org
(Difference between revisions)
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! Number !! Part !! Content !! Plasmid !! Length (bp) | ! Number !! Part !! Content !! Plasmid !! Length (bp) | ||
|- | |- | ||
| - | | #1 || J23119 || c.Promoter (Strong) || pSB1A2 || 35 | + | | #1 || J23119 || c.Promoter (Strong) || pSB1A2 || class="number" 35 |
|- | |- | ||
| - | | #2 || J23118 || c.Promoter (Medium) || BBa_J61002 || 35 | + | | #2 || J23118 || c.Promoter (Medium) || BBa_J61002 || class="number" 35 |
|- | |- | ||
| - | | #3 || B0032 || RBS || pSB1A2 || 13 | + | | #3 || B0032 || RBS || pSB1A2 || class="number" 13 |
|- | |- | ||
| - | | #5 || B0014 || d.Ter || pSB1AK3 || 95 | + | | #5 || B0014 || d.Ter || pSB1AK3 || class="number" 95 |
|- | |- | ||
| - | | #9 || E0240 || RBS-GFP-d.Ter || pSB1A2 || 876 | + | | #9 || E0240 || RBS-GFP-d.Ter || pSB1A2 || class="number" 876 |
|- | |- | ||
| - | | #10 || E0040 || GFP || pSB1A2 || 681 | + | | #10 || E0040 || GFP || pSB1A2 || class="number" 681 |
|- | |- | ||
| - | | #11 || E1010 || RFP || pSB2K3 || 723 | + | | #11 || E1010 || RFP || pSB2K3 || class="number" 723 |
|- | |- | ||
| - | | #14 || I712019 || fLuc || pSB1AK8 || 1653 | + | | #14 || I712019 || fLuc || pSB1AK8 || class="number" 1653 |
|- | |- | ||
| - | | #17 || J52008 || rLuc || pSB1AK3 || 936 | + | | #17 || J52008 || rLuc || pSB1AK3 || class="number" 936 |
|- | |- | ||
| - | | #20 || R0011 || lacP || pSB1A2 || 55 | + | | #20 || R0011 || lacP || pSB1A2 || class="number" 55 |
|- | |- | ||
| - | | #21 || C0012 || LacI || pSB1A2 || 1128 | + | | #21 || C0012 || LacI || pSB1A2 || class="number" 1128 |
|- | |- | ||
| - | | #22 || I712074 || pT7 || pSB1AK8 || 46 | + | | #22 || I712074 || pT7 || pSB1AK8 || class="number" 46 |
|- | |- | ||
| - | | #23 || K145001 || T7 RNA Pol. || pSB1A2 || 2655 | + | | #23 || K145001 || T7 RNA Pol. || pSB1A2 || class="number" 2655 |
|- | |- | ||
| - | | #24 || J22106 || recAp || pSB1A2 || 192 | + | | #24 || J22106 || recAp || pSB1A2 || class="number" 192 |
|- | |- | ||
| - | | #27 || C0083 || AspA || pSB2K3 || 1518 | + | | #27 || C0083 || AspA || pSB2K3 || class="number" 1518 |
|- | |- | ||
| - | | #28 || K112808 || T4 phage lysis device (no promoter) || pSB1A2 || 1785 | + | | #28 || K112808 || T4 phage lysis device (no promoter) || pSB1A2 || class="number" 1785 |
|- | |- | ||
| - | | #29 || - || CheZ || pSB1AK3 || 728 | + | | #29 || - || CheZ || pSB1AK3 || class="number" 728 |
|- | |- | ||
| - | | #30 || K117000 || Lysis gene || pSB1A2 || 144 | + | | #30 || K117000 || Lysis gene || pSB1A2 || class="number" 144 |
|- | |- | ||
| - | | #31 || - || LexA || pSB1AK3 || 750 | + | | #31 || - || LexA || pSB1AK3 || class="number" 750 |
|- | |- | ||
| - | | #33 || - || sulAp || pSB1AK3 || 67 | + | | #33 || - || sulAp || pSB1AK3 || class="number" 67 |
|- | |- | ||
| - | | #34 || - || uvrAp || pSB1AK3 || 96 | + | | #34 || - || uvrAp || pSB1AK3 || class="number" 96 |
|- | |- | ||
| #35 || - || recNp || || | | #35 || - || recNp || || | ||
|- | |- | ||
| - | | #36 || R0051 || cI-repressed promoter || pSB1A2 || 49 | + | | #36 || R0051 || cI-repressed promoter || pSB1A2 || class="number" 49 |
|- | |- | ||
| #37 || C0051 || cI repressor (LVA tagged) || pSB1A2 || \ | | #37 || C0051 || cI repressor (LVA tagged) || pSB1A2 || \ | ||
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|} | |} | ||
<!-- | <!-- | ||
| - | | #2-9 || || c.Promoter (M)-RBS-GFP-d.Ter || pSB1A2 || 911 | + | | #2-9 || || c.Promoter (M)-RBS-GFP-d.Ter || pSB1A2 || class="number" 911 |
|- | |- | ||
| - | | #20-9 || || lacP-RBS-GFP-d.Ter || pSB1A2 || 931 | + | | #20-9 || || lacP-RBS-GFP-d.Ter || pSB1A2 || class="number" 931 |
|- | |- | ||
| - | | #22-9 || || pT7-RBS-GFP-d.Ter || pSB1AK8 || 922 | + | | #22-9 || || pT7-RBS-GFP-d.Ter || pSB1AK8 || class="number" 922 |
|- | |- | ||
| - | | #3-11 || || RBS-RFP || pSB1A2 || 736 | + | | #3-11 || || RBS-RFP || pSB1A2 || class="number" 736 |
|- | |- | ||
| - | | #3-11-5 || || RBS-RFP-d.Ter || pSB1AK3 || 831 | + | | #3-11-5 || || RBS-RFP-d.Ter || pSB1AK3 || class="number" 831 |
|- | |- | ||
| - | | || || || pSB1C3 || 831 | + | | || || || pSB1C3 || class="number" 831 |
|- | |- | ||
| - | | #2-3-11-5 || || c.Promoter(M)-RBS-RFP-d.Ter || pSB1A2 || 866 | + | | #2-3-11-5 || || c.Promoter(M)-RBS-RFP-d.Ter || pSB1A2 || class="number" 866 |
|- | |- | ||
| - | | || || || pSB1AK3 || 866 | + | | || || || pSB1AK3 || class="number" 866 |
|- | |- | ||
| - | | #24-3-11-5 || || recAp-RBS-RFP-d.Ter || pSB1A2 || 1023 | + | | #24-3-11-5 || || recAp-RBS-RFP-d.Ter || pSB1A2 || class="number" 1023 |
|- | |- | ||
| - | | #33-3-11-5 || || sulAp-RBS-RFP-d.Ter || pSB1AK3 || 898 | + | | #33-3-11-5 || || sulAp-RBS-RFP-d.Ter || pSB1AK3 || class="number" 898 |
|- | |- | ||
| - | | #14-5 || || fLuc-d.Ter || pSB1AK3 || 1748 | + | | #14-5 || || fLuc-d.Ter || pSB1AK3 || class="number" 1748 |
|- | |- | ||
| - | | #3-14-5 || || RBS-ｆLuc-d.Ter || pSB1A2 || 1761 | + | | #3-14-5 || || RBS-ｆLuc-d.Ter || pSB1A2 || class="number" 1761 |
|- | |- | ||
| - | | #2-3-14-5 || || c.Promoter (M)-RBS-fLuc-d.Ter || pSB1A2 || 1796 | + | | #2-3-14-5 || || c.Promoter (M)-RBS-fLuc-d.Ter || pSB1A2 || class="number" 1796 |
|- | |- | ||
| - | | #20-3-14-5 || || lacP-RBS-fLuc-d.Ter || pSB1A2 || 1816 | + | | #20-3-14-5 || || lacP-RBS-fLuc-d.Ter || pSB1A2 || class="number" 1816 |
|- | |- | ||
| - | | #34-3-14-5 || || uvrAp-RBS-fLuc-d.Ter || pSB1AK3 || 1857 | + | | #34-3-14-5 || || uvrAp-RBS-fLuc-d.Ter || pSB1AK3 || class="number" 1857 |
|- | |- | ||
| - | | #36-3-14-5 || || cIp-RBS-fLuc-d.Ter || pSB1A2 || 1810 | + | | #36-3-14-5 || || cIp-RBS-fLuc-d.Ter || pSB1A2 || class="number" 1810 |
|- | |- | ||
| - | | #17-5 || || ｒLuc-d.Ter || pSB1AK3 || 1031 | + | | #17-5 || || ｒLuc-d.Ter || pSB1AK3 || class="number" 1031 |
|- | |- | ||
| - | | #3-17-5 || || RBS-ｒLuc-d.Ter || pSB1A2 || 1044 | + | | #3-17-5 || || RBS-ｒLuc-d.Ter || pSB1A2 || class="number" 1044 |
|- | |- | ||
| - | | #2-3-17-5 || || c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || 1079 | + | | #2-3-17-5 || || c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || class="number" 1079 |
|- | |- | ||
| - | | #24-3-17-5 || || recAp-RBS-rLuc-d.Ter || pSB1A2 || 1236 | + | | #24-3-17-5 || || recAp-RBS-rLuc-d.Ter || pSB1A2 || class="number" 1236 |
|- | |- | ||
| - | | #34-3-17-5 || || uvrAp-RBS-rLuc-d.Ter || pSB1AK3 || 1140 | + | | #34-3-17-5 || || uvrAp-RBS-rLuc-d.Ter || pSB1AK3 || class="number" 1140 |
|- | |- | ||
| - | | #20-3-17-5 || || lacP-RBS-ｒLuc-d.Ter || pSB1A2 || 1099 | + | | #20-3-17-5 || || lacP-RBS-ｒLuc-d.Ter || pSB1A2 || class="number" 1099 |
|- | |- | ||
| - | | #3-14-5-2-3-17-5 || || RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || 2840 | + | | #3-14-5-2-3-17-5 || || RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || class="number" 2840 |
|- | |- | ||
| - | | #20-3-14-5-2-3-17-5 || || lacP-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || 2895 | + | | #20-3-14-5-2-3-17-5 || || lacP-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || class="number" 2895 |
|- | |- | ||
| - | | #36-3-14-5-2-3-17-5 || || cIp-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || 2889 | + | | #36-3-14-5-2-3-17-5 || || cIp-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter || pSB1A2 || class="number" 2889 |
|- | |- | ||
| - | | #3-27 || || RBS-AspA || pSB1A2 || 1531 | + | | #3-27 || || RBS-AspA || pSB1A2 || class="number" 1531 |
|- | |- | ||
| - | | #3-27-5 || || RBS-AspA-d.Ter || pSB1AK3 || 1626 | + | | #3-27-5 || || RBS-AspA-d.Ter || pSB1AK3 || class="number" 1626 |
|- | |- | ||
| - | | #20-3-27-5 || || lacP-RBS-AspA-d.Ter || pSB1A2 || 1681 | + | | #20-3-27-5 || || lacP-RBS-AspA-d.Ter || pSB1A2 || class="number" 1681 |
|- | |- | ||
| - | | #3-29 || || RBS-CheZ || pSB1A2 || 741 | + | | #3-29 || || RBS-CheZ || pSB1A2 || class="number" 741 |
|- | |- | ||
| - | | #3-29-5 || || RBS-CheZ-d.Ter || pSB1AK3 || 836 | + | | #3-29-5 || || RBS-CheZ-d.Ter || pSB1AK3 || class="number" 836 |
|- | |- | ||
| - | | #20-3-29-5 || || lacP-RBS-CheZ-d.Ter || pSB1A2 || 891 | + | | #20-3-29-5 || || lacP-RBS-CheZ-d.Ter || pSB1A2 || class="number" 891 |
|- | |- | ||
| - | | #36-3-29-5 || || cIp-RBS-CheZ-d.Ter || pSB1A2 || 885 | + | | #36-3-29-5 || || cIp-RBS-CheZ-d.Ter || pSB1A2 || class="number" 885 |
|- | |- | ||
| #37-5 || || cI-d.Ter || pSB1AK3 || #VALUE! | | #37-5 || || cI-d.Ter || pSB1AK3 || #VALUE! | ||
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| #20-3-37-5 || || lacP-RBS-cI-d.Ter || pSB1A2 || #VALUE! | | #20-3-37-5 || || lacP-RBS-cI-d.Ter || pSB1A2 || #VALUE! | ||
|- | |- | ||
| - | | #20-28 || || lacP-〈T4 phage lysis device〉 || pSB1A2 || 1840 | + | | #20-28 || || lacP-〈T4 phage lysis device〉 || pSB1A2 || class="number" 1840 |
|- | |- | ||
| - | | #3-30 || || RBS-Lysis gene || pSB1A2 || 157 | + | | #3-30 || || RBS-Lysis gene || pSB1A2 || class="number" 157 |
|- | |- | ||
| - | | #3-23 || || RBS-T7 RNA Pol. || pSB1A2 || 2668 | + | | #3-23 || || RBS-T7 RNA Pol. || pSB1A2 || class="number" 2668 |
|- | |- | ||
| - | | pSB1A2 || || Plasmid backbone || pSB1A2 || 2079 | + | | pSB1A2 || || Plasmid backbone || pSB1A2 || class="number" 2079 |
|- | |- | ||
| - | | pSB1AK3 || || Plasmid backbone || pSB1AK3 || 3189 | + | | pSB1AK3 || || Plasmid backbone || pSB1AK3 || class="number" 3189 |
|- | |- | ||
| - | | pSB1A3 || || Linearized plasmid backbone || pSB1A3 || 2155 | + | | pSB1A3 || || Linearized plasmid backbone || pSB1A3 || class="number" 2155 |
|- | |- | ||
| - | | pSB1K3 || || Linearized plasmid backbone || pSB1K3 || 2204 | + | | pSB1K3 || || Linearized plasmid backbone || pSB1K3 || class="number" 2204 |
|- | |- | ||
| - | | pSB1C3 || || Linearized plasmid backbone || pSB1C3 || 2070 | + | | pSB1C3 || || Linearized plasmid backbone || pSB1C3 || class="number" 2070 |
|- | |- | ||
| - | | pSB1C3 || J04450 || lacP-RBS-RFP-d.Ter || pSB1C3 || 1069 | + | | pSB1C3 || J04450 || lacP-RBS-RFP-d.Ter || pSB1C3 || class="number" 1069 |
|} | |} | ||
--> | --> | ||
Revision as of 20:52, 13 September 2011
Lab Note
iGEM UT-Tokyo
Parts List
| Number | Part | Content | Plasmid | Length (bp) |
|---|---|---|---|---|
| #1 | J23119 | c.Promoter (Strong) | pSB1A2 | class="number" 35 |
| #2 | J23118 | c.Promoter (Medium) | BBa_J61002 | class="number" 35 |
| #3 | B0032 | RBS | pSB1A2 | class="number" 13 |
| #5 | B0014 | d.Ter | pSB1AK3 | class="number" 95 |
| #9 | E0240 | RBS-GFP-d.Ter | pSB1A2 | class="number" 876 |
| #10 | E0040 | GFP | pSB1A2 | class="number" 681 |
| #11 | E1010 | RFP | pSB2K3 | class="number" 723 |
| #14 | I712019 | fLuc | pSB1AK8 | class="number" 1653 |
| #17 | J52008 | rLuc | pSB1AK3 | class="number" 936 |
| #20 | R0011 | lacP | pSB1A2 | class="number" 55 |
| #21 | C0012 | LacI | pSB1A2 | class="number" 1128 |
| #22 | I712074 | pT7 | pSB1AK8 | class="number" 46 |
| #23 | K145001 | T7 RNA Pol. | pSB1A2 | class="number" 2655 |
| #24 | J22106 | recAp | pSB1A2 | class="number" 192 |
| #27 | C0083 | AspA | pSB2K3 | class="number" 1518 |
| #28 | K112808 | T4 phage lysis device (no promoter) | pSB1A2 | class="number" 1785 |
| #29 | - | CheZ | pSB1AK3 | class="number" 728 |
| #30 | K117000 | Lysis gene | pSB1A2 | class="number" 144 |
| #31 | - | LexA | pSB1AK3 | class="number" 750 |
| #33 | - | sulAp | pSB1AK3 | class="number" 67 |
| #34 | - | uvrAp | pSB1AK3 | class="number" 96 |
| #35 | - | recNp | ||
| #36 | R0051 | cI-repressed promoter | pSB1A2 | class="number" 49 |
| #37 | C0051 | cI repressor (LVA tagged) | pSB1A2 | \ |
| #38 | - | RecA |
Lab Diary
For convenience sake, each part (gene, promotors, etc) are represented by consecutive numbers (e.g. #20 for lac promotor). By pointing the mouse on the part number in the labnote, you can find out the details of the part.
