Team:Washington/Alkanes/Methods

From 2011.igem.org

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INSERT INFO HERE... LOOK AT OTHER PAGES FOR EXAMPLES OF FORMATING AND INSERTING PICTURES.  CLICK THE EDIT BUTTON ON THE UPPER LEFT SIDE OF THE PAGE AFTER YOU HAVE SIGNED IN.
 
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In order to produce alkanes, we need both Acyl-ACP Reductase and Aldehyde Decarbonylase to work together in the cells, which is the basic system.
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=Basic Alkane Production Construct=
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Insert description and WikiDust figure here
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[[File:Allkane_groups.png‎ |right|250px|thumb|]]
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=Alkane Production=
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=Building a Perfect Alkane Production System=
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Outline basic growth and extraction procedure... basically graphics from presentation with added text
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Our goal is not only to build a system that produces alkane with Acyl-ACP Reductase and Aldehyde Decarbonylase, but also to modify the whole system on producing shorter length of alkanes, higher yield of production of alkanes, higher quality of alkanes (branched alkanes), etc. In order to achieve all these, we have groups work on six different directions: system optimization, alternative chassis, decarbonylase redesign, alternative aldehyde, enzyme localization, branched alkanes.
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===System Optimization===
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=Alkane Detection=
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Single plasmid vs. double plasmid.
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Explain GCMS... again basically graphics from presentation with text
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===alternative chassis===
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The idea of alternative chassis is to run the basic system on different types vectors.
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===decarbonylase redesign===
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===alternative aldehyde===
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===enzyme localization===
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===branched alkanes===
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=System Performance Under Different Conditions=
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===Plastic Vs. Glass===
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===Microaerobic Vs. Aerobic===
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=Testing Productions With GCMS=
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Revision as of 16:30, 12 September 2011


Basic Alkane Production Construct

Insert description and WikiDust figure here

Alkane Production

Outline basic growth and extraction procedure... basically graphics from presentation with added text

Alkane Detection

Explain GCMS... again basically graphics from presentation with text

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